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Soil microbial community analysis to identify syntrophic relationships between microbes

Research Project

Project/Area Number 24770015
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionTokyo Institute of Technology

Principal Investigator

MORI Hiroshi  東京工業大学, 生命理工学研究科, 助教 (40610837)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細菌群集 / 代謝 / メタゲノム / バイオインフォマティクス / 生態
Outline of Final Research Achievements

We conducted a bioinformatics analysis of a time-series metagenomic sequencing data of a soil microbial community with the aromatic compounds pollution. Developing and using the bioinformatics method to efficiently identify fluctuating genes and taxa in time-series metagenomic data, we identified a catalog of fluctuating taxa and genes during aromatic compounds degradation in the soil. In addition, we developed a tool VITCOMIC2 which can (i) identify 16S rRNA gene sequences in metagenome sequence data with minimum false positive rate, (ii) assign taxonomy of the sequences, and (iii) calculate taxonomic composition of the sample.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (6 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe.2015

    • Author(s)
      Wang T, Mori H, Zhang C, Kurokawa K, Xing X, Yamada T
    • Journal Title

      BMC Bioinformatics

      Volume: 16 Issue: 1 Pages: 96-96

    • DOI

      10.1186/s12859-015-0499-y

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CLAST: CUDA implemented large-scale alignment search tool.2014

    • Author(s)
      Yano M, Mori H, Akiyama Y, Yamada T, Kurokawa K
    • Journal Title

      BMC Bioinformatics

      Volume: 15 Issue: 1 Pages: 406-406

    • DOI

      10.1186/s12859-014-0406-y

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes2013

    • Author(s)
      Mori H, Maruyama F, Kato H, Toyoda A, Dozono A, Ohtsubo Y, Nagata Y, Fujiyama A, Tsuda M Kurokawa K
    • Journal Title

      DNA Res

      Volume: 21 Issue: 2 Pages: 217-227

    • DOI

      10.1093/dnares/dst052

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meeting Report: Hackathon-Workshop on Darwin Core and MIxS Standards Alignment (February 2012).2012

    • Author(s)
      Tuama, EO., et al.
    • Journal Title

      Stand Genomic Sci.

      Volume: 7 Issue: 1 Pages: 166-170

    • DOI

      10.4056/sigs.3166513

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Current and future trends in metagenomics : Development of knowledge bases2012

    • Author(s)
      森宙史, 他
    • Journal Title

      Journal of Information Processing and Management

      Volume: 55 Issue: 3 Pages: 167-175

    • DOI

      10.1241/johokanri.55.167

    • NAID

      130001855927

    • ISSN
      0021-7298, 1347-1597
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] VITCOMIC2 analysis system: visualization tool for taxonomic composition of microbial communities2015

    • Author(s)
      Mori H, Maruyama T, Yano M, Yamada T, Kurokawa K
    • Organizer
      International Symposium on Genome Science 2015
    • Place of Presentation
      Hitotsubashi Hall, Tokyo, Japan
    • Year and Date
      2015-01-20 – 2015-01-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Design and experimental validation of a novel non-degenerate universal primer set for 16S rRNA gene amplicon sequencing with a low possibility to amplify eukaryotic rRNA genes2013

    • Author(s)
      Hiroshi Mori, Fumito Maruyama, Hiromi Kato, Atsushi Toyoda, Ayumi Dozono, Yoshiyuki Ohtsubo, Yuji Nagata, Asao Fujiyama, Masataka Tsuda, and Ken Kurokawa
    • Organizer
      2nd Thunen Symposium on Soil Metagenomics
    • Place of Presentation
      Thunen Institute, Braunschweig, Germany
    • Related Report
      2013 Research-status Report
  • [Presentation] メタゲノムインフォマティクスと微生物統合DB

    • Author(s)
      森宙史
    • Organizer
      第41回GCOE談話会
    • Place of Presentation
      東京大学情報生命科学実験棟2階講義室
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 微生物の生息環境データの記述および高度な検索のためのオントロジー構築

    • Author(s)
      森宙史, 他
    • Organizer
      トーゴーの日シンポジウム2012
    • Place of Presentation
      時事通信ホール, 東京
    • Related Report
      2012 Research-status Report
  • [Presentation] メタゲノム解析を用いた細菌群集動態の解明

    • Author(s)
      森宙史
    • Organizer
      第195回信州大学理学部生物科教室セミナー
    • Place of Presentation
      信州大学松本キャンパス, 長野
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 微生物統合データベース MicrobeDB.jp

    • Author(s)
      森宙史, 他
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学, 滋賀
    • Related Report
      2012 Research-status Report
  • [Book] メタゲノミクスの現状と未来2014

    • Author(s)
      山本希, 森宙史, 山田拓司, 黒川顕
    • Total Pages
      10
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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