| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Upon herbivore attack, plants respond to elicitors included in saliva injected. Despite the characterization of several insect salivary elicitors in the last decade, none of pattern recognition receptors have been identified so far. In the current study, we assessed 16 soybean genes that are homologous to an Arabidopsis CERK1 (Chitin Elicitor Receptor Kinase) gene, in order to isolate and characterize Herbivore Elicitor Receptor Kinase (HERK), involved in the recognition of oral secretions (OS) from Spodoptera litura larvae as ligand.
We produced transgenic Arabidopsis lines, expressing the respective of CERK1-like kinase. In leaves treated with mechanical wounding plus S. litura OS, the five transgenic lines induced the expression of a defense gene PDF1.2, more drastically than that in the wild-type plants. We will reveal the molecular mechanisms underling the GmCLK-based elicitor recognition in plants.
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