| Project/Area Number |
24770033
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
HOSHINO Tatsuhiko 独立行政法人海洋研究開発機構, 高知コア研究所, 研究員 (30386619)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 地下生命圏 / 炭素フラックス / 一酸化炭素 / 機能遺伝子 / 微生物生態 / 一酸化炭素酸化 / 海底下 / 堆積物 / 炭素循環 |
|---|
| Research Abstract |
In the past decades, microbial community in the marine subsurface environments have been studied, and total biomass of microbial cells in the marine subsurface was estimated to contribute to 0.6% of the total living biomass carbon on the Earth. However, roles of those deep life were still largely unknown. In this study, we first investigated diversity of cooS gene, which encodes beta subunit of anaerobic carbon monoxide dehydrogenase, in the subseafloor environments. DNA was extracted from marine sediments obtained at Gulf of Mexico, Fuan de Fuca, South Pacific Gyre, Nankai Trough, and off Shimokita peninsula by IODP expeditions. Fragment of cooS gene was amplified by PCR and sequenced. The sequencing results indicated that diversity of cooS gene in the sediments were very small comparing to 16S rRNA gene. Almost all obtained sequences were not related to the known cooS gene sequences in the database and formed distinct clusters in a phylogenetic tree.
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