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Analysis of plastid differentiation by chromatin immunoprecipitation

Research Project

Project/Area Number 24770037
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionChiba University

Principal Investigator

HANAOKA MITSUMASA  千葉大学, 園芸学研究科, 准教授 (30508122)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords色素体分化 / 葉緑体 / ChIP法 / シグマ因子 / T87細胞 / 色素体 / 転写制御 / 分化 / クロマチン免疫沈降法 / シロイヌナズナ / 培養細胞
Research Abstract

The purpose of this study is to further clarify molecular mechanisms of plastid differentiation by the use of chromatin immunoprecipitation (ChIP)-based method. We here established a novel induction system of chloroplast differentiation using Arabidopsis thaliana T87 suspension cultured cells, and examined functional roles of plastid sigma factors during chloroplast differentiation. Furthermore, transcriptional regulation during amyloplast differentiation has been also characterized.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (55 results)

All 2014 2013 2012 Other

All Journal Article (17 results) (of which Peer Reviewed: 14 results) Presentation (35 results) (of which Invited: 3 results) Remarks (3 results)

  • [Journal Article] The circadian regulation of photosynthesis.2014

    • Author(s)
      Dodd AN, Kusakina J, Hall A, Gould PD, Hanaoka M.
    • Journal Title

      Photosynth Res.

      Volume: 119 Issue: 1-2 Pages: 181-190

    • DOI

      10.1007/s11120-013-9811-8

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 植物が葉緑体に「時」の情報を伝えるメカニズム2014

    • Author(s)
      華岡光正
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: Vol. 72, No. 2 Pages: 125-126

    • URL

      http://www.jba.or.jp/pc/archive/2014/vol72_no2.html

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes.2014

    • Author(s)
      Shin MR, Natsuume M, Matsumoto T, Hanaoka M, Imai M, Iijima K, Oka S, Adachi E, Kodama H.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 2 Pages: e87869-e87869

    • DOI

      10.1371/journal.pone.0087869

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circadian control of chloroplast transcription by a nuclear-encoded timing signal.2013

    • Author(s)
      Noordally ZB, Ishii K, Atkins KA, Wetherill SJ, Kusakina J, Walton EJ, Kato M, Azuma M, Tanaka K, Hanaoka M, Dodd AN.
    • Journal Title

      Science

      Volume: 339 Issue: 6125 Pages: 1316-1319

    • DOI

      10.1126/science.1230397

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ChIP法を用いた葉緑体光合成遺伝子の転写制御解析2013

    • Author(s)
      華岡光正
    • Journal Title

      光合成研究

      Volume: Vol. 23, No. 3 Pages: 107-110

    • NAID

      40019934270

    • URL

      http://photosyn.jp/journal/kaiho68.pdf

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of four nuclear-encoded plastid RNA polymerase sigma factor genes in the liverwort Marchantia polymorpha: blue-light- and multiple stress-responsive SIG5 was acquired early in the emergence of terrestrial plants.2013

    • Author(s)
      Kanazawa T, Ishizaki K, Kohchi T, Hanaoka M, Tanaka K.
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 10 Pages: 1736-1748

    • DOI

      10.1093/pcp/pct119

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear-encoded chloroplast RNA polymerase sigma factor SIG2 activates chloroplast-encoded phycobilisome genes in a red alga, Cyanidioschyzon merolae.2013

    • Author(s)
      Fujii G, Imamura S, Hanaoka M, Tanaka K.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 20 Pages: 3354-3359

    • DOI

      10.1016/j.febslet.2013.08.031

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The early days of plastid retrograde signaling with respect to replication and transcription2013

    • Author(s)
      Kan Tanaka
    • Journal Title

      Front. Plant Physiol.

      Volume: 3 Pages: 1-5

    • DOI

      10.3389/fpls.2012.00301

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 24時間リズムを生み出す遺伝子発現調節機構2013

    • Author(s)
      華岡光正
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: Vol. 71, No. 1 Pages: 36-37

    • NAID

      10031140300

    • URL

      http://www.jba.or.jp/pc/archive/2013/vol71_no1.html

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Mitochondrial localization of ferrochelatase in a red alga Cyanidioschyzon merolae2013

    • Author(s)
      S. Watanabe , M. Hanaoka, M. Ohnuma, S. Taketani and K.Tanaka
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 8 Pages: 1289-1295

    • DOI

      10.1093/pcp/pct077

    • NAID

      10031195182

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 単細胞紅藻シゾンにおける光応答:葉緑体自律的な転写制御2013

    • Author(s)
      華岡光正
    • Journal Title

      BSJ-Review「植物科学の最前線」

      Volume: 4 Pages: 13-20

    • Related Report
      2012 Research-status Report
  • [Journal Article] The circadian regulation of photosynthesis.2013

    • Author(s)
      Antony N. Dodd
    • Journal Title

      Photosynthesis Research

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] SIG1, a sigma factor for the chloroplast RNA polymerase, differentially associates with multiple DNA regions in the chloroplast chromosomes in vivo2012

    • Author(s)
      Mitsumasa Hanaoka
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 13 Issue: 10 Pages: 12182-12194

    • DOI

      10.3390/ijms131012182

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear-encoded plastid sigma factor SIG6 exclusively contributes to chloroplast differentiation in plastid differentiation of Arabidopsis thaliana2012

    • Author(s)
      Shunsuke Hirooka, Mitsumasa Hanaoka, Kazuhiko Enami, Takehiko Kanazawa, Toshiyuki Sone, Yuta Imoto, Akikazu Ando, Haruko Kuroiwa, Tsuneyoshi Kuroiwa and Kan Tanaka
    • Journal Title

      Cytologia

      Volume: 77 (1) Pages: 73-82

    • NAID

      10030120114

    • URL

      https://www.jstage.jst.go.jp/article/cytologia/77/1/77_73/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Retrograde signals arise from reciprocal crosstalk within plastids2012

    • Author(s)
      Kazuhiko Enami
    • Journal Title

      Plant Signal.Behav.

      Volume: 7 Issue: 1 Pages: 142-144

    • DOI

      10.4161/psb.7.1.18451

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear-encoded plastid sigma factor SIG6 exclusively contributes to chloroplast differentiation in plastid differentiation of Arabidopsis thaliana.2012

    • Author(s)
      Shunsuke Hirooka
    • Journal Title

      Cytologia

      Volume: 77 Pages: 73-82

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RpaB, another response regulator operating circadian clock-dependent transcriptional regulation in Synechococcus elongat us PCC 7942.2012

    • Author(s)
      Hanaoka M, Takai N, Hosokawa N, Fujiwara M, Akimoto Y, Kobori N, Iwasaki H, Kondo T, Tanaka K.
    • Journal Title

      J Biol Chem

      Volume: 287(31) Issue: 31 Pages: 26321-7

    • DOI

      10.1074/jbc.m111.338251

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナT87培養細胞の葉緑体分化に際した転写制御の解析2014

    • Author(s)
      木山貴史, 江波和彦, 上原浩一, 華岡光正
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      東京
    • Year and Date
      2014-03-30
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナ培養細胞におけるアミロプラスト分化機構の解析2014

    • Author(s)
      恩田和幸, 江波和彦, 華岡光正
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 概日リズム情報の共有に関わる核と葉緑体間のシグナル伝達2014

    • Author(s)
      石井健雄, 加藤麻衣子, Zeenat B. Noordally, Kelly A. Atkins, Jelena Kusakina, 東美由紀, 田中寛, Antony N. Dodd, 華岡光正
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Year and Date
      2014-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体分化過程におけるプラスチドシグナルに依存した遺伝子発現制御2013

    • Author(s)
      江波和彦, 木山貴史, 上原浩一, 華岡光正
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Endosymbiotic evolution of light responses in chloroplasts2013

    • Author(s)
      Mitsumasa Hanaoka, Takayuki Kawakami, Daichi Satoh, Hiroyuki Ando, Misato Anma, Gaku Fujii, Sousuke Imamura and Kan Tanaka
    • Organizer
      The 12th International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Halifax, Canada
    • Year and Date
      2013-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles of the possible photosensory two-component system in the chloroplasts of Cyanidioschyzon merolae2013

    • Author(s)
      Mitsumasa Hanaoka, Takayuki Kawakami, Daichi Satoh, Hiroyuki Ando, Gaku Fujii, Sousuke Imamura and Kan Tanaka
    • Organizer
      International Symposium of Plant Photobiology (ISPP) 2013 conference
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2013-06-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体の分化・発達に伴った光合成遺伝子の転写制御2013

    • Author(s)
      安間美里, 木山貴史, 加藤麻衣子, 江波和彦, 田中寛, 華岡光正
    • Organizer
      第4回光合成学会・シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2013-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 概日時計シグナルによる葉緑体遺伝子の転写調節2013

    • Author(s)
      石井健雄, 加藤麻衣子, Zeenat B. Noordally, Kelly A. Atkins, Jelena Kusakina, 東美由紀, 田中寛, Antony N. Dodd, 華岡光正
    • Organizer
      第4回光合成学会・シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2013-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] アミロプラスト分化における核遺伝子の発現制御2013

    • Author(s)
      恩田和幸, 江波和彦, 華岡光正
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナT87細胞を用いた葉緑体分化における遺伝子発現制御の解析2013

    • Author(s)
      江波和彦, 木山貴史, 安間美里, 加藤麻衣子, 田中寛, 華岡光正
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] シアノバクテリアの概日時計機構を支えるRpaA/Bによる協調的な転写制御2012

    • Author(s)
      華岡光正, 高井直樹, 細川徳宗, 藤原正幸, 秋元勇輝, 小堀奈美, 岩崎秀雄, 近藤孝男, 田中寛
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2012-12-06
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナT87細胞を用いた葉緑体分化誘導系の構築と葉緑体遺伝子の転写制御の解析2012

    • Author(s)
      木山貴史, 江波和彦, 加藤麻衣子, 安間美里, 田中寛, 華岡光正
    • Organizer
      日本農芸化学会関東支部2012年度大会
    • Place of Presentation
      新潟
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] ChIP法によるシロイヌナズナの葉緑体分化に伴った転写制御機構の解析2012

    • Author(s)
      華岡光正, 加藤麻衣子, 安間美里, 木山貴史, 江波和彦, 田中寛
    • Organizer
      第76回日本植物学会大会
    • Place of Presentation
      姫路
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] RpaB is involved in transcriptional regulation in response to the circadian clock as well as high light stress in Synechococcus elongatus PCC 79422012

    • Author(s)
      Mitsumasa Hanaoka, Naoki Takai, Norimune Hosokawa, Masayuki Fujiwara, Yuki Akimoto, Nami Kobori, Hideo Iwasaki, Takao Kondo and Kan Tanaka
    • Organizer
      ISPP 2012: The 14th International symposium on Phototrophic Prokaryotes
    • Place of Presentation
      Porto, Portugal
    • Year and Date
      2012-08-07
    • Related Report
      2013 Final Research Report
  • [Presentation] シアノバクテリアの概日時計応答に関わる新たな転写制御様式の発見2012

    • Author(s)
      華岡光正, 高井直樹, 細川徳宗, 藤原正幸, 秋元勇輝, 小堀奈美, 岩崎秀雄, 近藤孝男, 田中寛
    • Organizer
      第3回光合成学会・シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体の分化・発達に伴った光合成遺伝子の転写制御

    • Author(s)
      安間美里、木山貴史、加藤麻衣子、江波和彦、田中寛、華岡光正
    • Organizer
      第4回光合成学会・シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 概日時計シグナルによる葉緑体遺伝子の転写調節

    • Author(s)
      石井健雄、加藤麻衣子、Zeenat B. Noordally、Kelly A. Atkins、Jelena Kusakina、東美由紀、田中寛、Antony N. Dodd、華岡光正
    • Organizer
      第4回光合成学会・シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Roles of the possible photosensory two-component system in the chloroplasts of Cyanidioschyzon merolae.

    • Author(s)
      Mitsumasa Hanaoka, Takayuki Kawakami, Daichi Satoh, Hiroyuki Ando, Gaku Fujii, Sousuke Imamura and Kan Tanaka
    • Organizer
      International Symposium of Plant Photobiology (ISPP) 2013 conference
    • Place of Presentation
      The University of Edinburgh, UK
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Endosymbiotic evolution of light responses in chloroplasts.

    • Author(s)
      Mitsumasa Hanaoka, Takayuki Kawakami, Daichi Satoh, Hiroyuki Ando, Misato Anma, Gaku Fujii, Sousuke Imamura and Kan Tanaka
    • Organizer
      The 12th International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Halifax University, Canada
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] RNAサイレンシングにともなうスプライシングへの干渉作用について

    • Author(s)
      申美來、松本貴嗣、華岡光正、今井美咲、飯島健、児玉浩明
    • Organizer
      第31回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンの葉緑体光応答と転写制御機構

    • Author(s)
      佐藤大地、安藤洸幸、小倉駿佑、藤井岳、今村壮輔、田中寛、華岡光正
    • Organizer
      第77回日本植物学会大会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 葉緑体分化過程におけるプラスチドシグナルに依存した遺伝子発現制御

    • Author(s)
      江波和彦、木山貴史、上原浩一、華岡光正
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 色素体分化と概日時計機構を支える核と葉緑体間のシグナル伝達

    • Author(s)
      華岡光正
    • Organizer
      第16回植物オルガネラワークショップ
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 概日リズム情報の共有に関わる核と葉緑体間のシグナル伝達

    • Author(s)
      石井健雄、加藤麻衣子、Zeenat B. Noordally、Kelly A. Atkins、Jelena Kusakina、東美由紀、田中寛、Antony N. Dodd、華岡光正
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナ培養細胞におけるアミロプラスト分化機構の解析

    • Author(s)
      恩田和幸、江波和彦、華岡光正
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cyanidioschyzon merolaeの光応答転写制御における二成分制御系の役割

    • Author(s)
      安藤洸幸、小倉駿佑、佐藤大地、藤井岳、今村壮輔、田中寛、五十嵐雅之、内海龍太郎、華岡光正
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナT87培養細胞の葉緑体分化に際した転写制御の解析

    • Author(s)
      木山貴史、江波和彦、上原浩一、華岡光正
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] シアノバクテリアの概日時計応答に関わる新たな転写制御様式の発見

    • Author(s)
      華岡光正
    • Organizer
      第3回光合成学会・シンポジウム
    • Place of Presentation
      東京工業大学(横浜)
    • Related Report
      2012 Research-status Report
  • [Presentation] RpaB is involved in transcriptional regulation in response to the circadian clock as well as high light stress in Synechococcus elongatus PCC 7942

    • Author(s)
      Mitsumasa Hanaoka
    • Organizer
      ISPP 2012: The 14th International symposium on Phototrophic Prokaryotes
    • Place of Presentation
      Porto Palacio(ポルトガル)
    • Related Report
      2012 Research-status Report
  • [Presentation] シアノバクテリアの概日時計出力系に関わる新規転写制御因子RpaBの役割

    • Author(s)
      華岡光正
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2012 Research-status Report
  • [Presentation] ChIP法によるシロイヌナズナの葉緑体分化に伴った転写制御機構の解析

    • Author(s)
      華岡光正
    • Organizer
      第76回日本植物学会大会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナT87細胞を用いた葉緑体分化誘導系の構築と葉緑体遺伝子の転写制御の解析

    • Author(s)
      木山貴史
    • Organizer
      日本農芸化学会関東支部2012年度大会
    • Place of Presentation
      新潟薬科大学(新潟)
    • Related Report
      2012 Research-status Report
  • [Presentation] シアノバクテリアの概日時計機構を支えるRpaA/Bによる協調的な転写制御

    • Author(s)
      華岡光正
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナT87細胞を用いた葉緑体分化における遺伝子発現制御の解析

    • Author(s)
      華岡光正
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report
  • [Presentation] アミロプラスト分化における核遺伝子の発現制御

    • Author(s)
      恩田和幸
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2012 Research-status Report
  • [Remarks] 千葉日報(朝刊)2013年3月15日「伝達の仕組み解明植物の"体内時計"情報」

    • Related Report
      2013 Final Research Report
  • [Remarks] Nature Japan特集記事 「よく似た2つの転写因子が正負の協調を行うことで、24時間の生体リズムを刻んでいた!」

    • URL

      http://www.natureasia.com/ja-jp/jobs/tokushu/detail/260

    • Related Report
      2013 Final Research Report
  • [Remarks] Science日本語版特集号 「核からの時間シグナルによる葉緑体転写の概日制御」

    • URL

      http://www.sciencemag.jp/files/2014-Issue_Science_JapanResearch-2013_72dpi.pdf

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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