Development of quantitative analysis methods of plant cells by image processing for microscope
| Project/Area Number |
24770038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 植物 / 細胞・組織 / 解析・評価 / 共焦点顕微鏡 / 生体生命情報学 |
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| Outline of Final Research Achievements |
Image processing methods of plant intra-cellular structures were developed to evaluate distribution, morphology, speed and co-localization through the categorizing of fluorescence image into particular, membraneous, lemenous and fiberous pattern. In addition, an algorithm based on machine learning technique was established to generate highly accurate classification software to estimate phase of cell cycle from fluorescence image of nuclei/chromosomes.
As a result, grounded by microscopic image analyses, quantitative insights were obtained in wide-ranging field of plant cytology including a regulatory mechanism of cortical microtubules, an effect of cold stress on endoplasmic streaming, a role of actin microfilaments in cell division and dynamic localization of proteins related to intra-cellular vesicle transport system.
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Report
(4 results)
Research Products
(97 results)
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[Journal Article] Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis.2014
Author(s)
Naramoto S, Otegui MS, Kutsuna N, de Rycke R, Dainobu T, Karampelias M, Fujimoto M, Feraru E, Miki D, Fukuda H, Nakano A, Friml J
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Journal Title
Plant Cell
Volume: 26
Issue: 7
Pages: 3062-1076
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] An atypical tubulin kinase mediates stress-induced microtubule depolymer ization in Arabidopsis2013
Author(s)
Satoshi Fujita, Jaromir Pytela, Takashi Hotta, Takehide Kato, Takahiro Hamada, Rie Akamatsu, Yasumasa Ishida, Natsumaro Kutsuna, Seiichiro Hasezawa, Yuko Nomura, Hirofumi Nakagami, Takashi Hashimoto
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Journal Title
Curr. Biol
Volume: 23
Issue: 20
Pages: 1969-1978
DOI
Related Report
Peer Reviewed
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[Presentation] Chemical phenomics for biomass engineering.2015
Author(s)
栗原(大窪)恵美子, 栗原志夫, 大谷美沙都, 朽名夏麿, 永田典子, 小林恵, 小松功典, 菊池淳, 掛川弘一, Ong WenDee, 出村拓, 松井南
Organizer
第56回日本植物生理学会年会
Place of Presentation
東京都 東京農業大学 世田谷キャンパス
Year and Date
2015-03-17
Related Report
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[Presentation] Radiocesium contamination in Satoyama forests in Kashiwa city, suburb of the Tokyo metropolitan area2014
Author(s)
Kenji Fukuda, Natsumaro Kutsuna, Toru Terada, Mohammad Reza Mansounia, Mohammad Nizam Uddin, Katsuaki Jimbo, Marcin Jarzebski, Sonomi Shibuya, Juri Fujieda, Hirokazu Yamamoto, Makoto Yokohari
Organizer
International Union of Forest Research Organizations (IUFRO) XXIV World Congress
Place of Presentation
The Salt Palace Convention Center in Salt Lake City, Utah, USA
Year and Date
2014-10-11
Related Report
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[Presentation] 植物の細胞内ダイナミクス2014
Author(s)
上田晴子, 横田悦雄, 朽名夏麿, 西村いくこ
Organizer
第23回日本バイオイメージング学会学術集会
Place of Presentation
大阪府吹田市 大阪大学銀杏会館
Year and Date
2014-09-06
Related Report
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[Presentation] The pathogenic modulation of host endosomes at the plant-pathogen interface2014
Author(s)
Noriko Inada, Shigeyuki Betsuyaku, Takashi L. Shimada, Kazuo Ebine, Emi Ito, Natsumaro Kutsuna, Seiichiro Hasezawa, Yoshitaka Takano, Hiroo Fukuda, Akihiko Nakano, Takashi Ueda
Organizer
XVI International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Rhodes, Greece
Year and Date
2014-07-09
Related Report
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[Presentation] 環境ストレスに対応する微小管制御
Author(s)
藤田智史, Jaromir Pytela, 堀田祟, 加藤壮英, 濱田隆宏, Wong Jeh-Haur, 朽名夏麿, 野村有子, 馳澤盛一郎, 中神弘史, 橋本隆
Organizer
日本植物学会第77回大会
Place of Presentation
北海道 北海道大学札幌キャンパス
Related Report
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[Presentation] 電顕アトラス: 高圧凍結技法を取り入れた広域電顕像撮影システムの開発
Author(s)
佐藤繭子, 朽名夏麿, 澤木史江, 若崎眞由美, 桧垣匠, 吉田拓広, 櫻井哲也1, 馳澤盛一郎2, 持田恵一, 永田典子, 豊岡公徳
Organizer
第54回日本植物生理学会年会
Place of Presentation
岡山県 岡山大学津島キャンパス
Related Report
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