Elucidation of the role of the vacuolar type proton pyrophosphatase in early stages of plant development
| Project/Area Number |
24770039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo Gakugei University |
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Principal Investigator |
FERJANI Ali 東京学芸大学, 教育学部, 准教授 (20530380)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | H+PPase / fugu5変異体 / 代謝制御 / ピロリン酸 / ショ糖 / 糖新生 / 補償作用 / 葉の発生 / H+-PPase / 葉器官サイズ制御 / 細胞増殖 / 細胞伸長 / 液胞プロトンピロホスファターゼ |
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| Research Abstract |
Following germination, oil-seed plants rely on triacylglycerol (TAG) reserves for growth during a heterotrophic period, where TAG is converted to sucrose (Suc). Also, macromolecular syntheses consume huge amounts of ATP, often hydrolyzed to AMP plus PPi. In Arabidopsis, the vacuolar H+-PPase uses energy from PPi hydrolysis to acidify the vacuole. The fugu5 mutants, lacking the AVP1 H+-PPase, failed to sustain postgerminative heterotrophic growth; recovered upon Suc supply, or specific PPi removal by the cytosolic PPase IPP1. Thus the major function of H+-PPase in seedling development is the removal of inhibitory PPi, rather than H+ pumping. Suc and PPi quantification in WT versus fugu5 indicated that gluconeogenesis is inhibited by elevated level of PPi, but the targets of PPi inhibition remained unclear. Here, profiling of major metabolites that occur during TAG mobilization showed that UDP-Glucose Pyrophosphorylase (UGPase) is specifically inhibited by excess cytosolic PPi in fugu5.
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Report
(3 results)
Research Products
(59 results)
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[Journal Article] Peptide Separation Methodologies for in-depth Proteomics in Arabidopsis.2013
Author(s)
Fukao, Y, Yoshida, M, Kurata, R, Kobayashi, M, Nakanishi, M, Fujiwara, M, Nakajima, K., and Feriani, A.
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Journal Title
Plant Cell Physiol.
Volume: (in press)
Issue: 5
Pages: 808-815
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] The ATM-dependent DNA damage response acts as an upstream trigger for compensation in the fas1 mutation during Arabidopsis leaf development2013
Author(s)
Hisagana T, Ferjani A, Horiguchi G, Ishikawa N, Fujikura U, Kubo M, Demura T, Fukuda H, Ishida T, Sugimoto K, Tsukaya H
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Journal Title
Plant Physiol.
Volume: 162(2)
Issue: 2
Pages: 831-841
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity.2013
Author(s)
Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M. and Tsukaya, H.
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Journal Title
Plant Cell Physiology
Volume: 54
Issue: 12
Pages: 1989-1998
DOI
Related Report
Peer Reviewed
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[Presentation] The metabolic target of pyrophosphate, a mysterious player in plant metabolism, identified2014
Author(s)
Ali Ferjani, Kensuke Kawade, Akira Oikawa, Mariko Asaoka, Kazuki Takahashi, Masanori Ishida, Masayoshi Maeshima, Masami Yokota Hirai, Kazuki Saito, Hirokazu Tsukaya
Organizer
25th International Conference on Arabidopsis Research
Place of Presentation
Canada, Vancouver
Related Report
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[Presentation] ATM-DEPENDENT DNA DAMAGE RESPONSE COORDINATES CELL PROLIFERATION AND CELL EXPANSION IN ARABIDOPSIS LEAF GROWTH2012
Author(s)
Tetsuya Hisanaga, Ali Ferjani, Gorou Horiguchi, Naoko Ishikawa, Ushio Fujikura, Minoru Kubo, Taku Demura, Hiroo Fukuda, Takashi Ishida, Keiko Sugimoto and Hirokazu Tsukaya
Organizer
23rd International Conference on Arabidopsis Research
Place of Presentation
Austria, Vienna
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