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Elucidation of thioredoxin mediated redox-regulation of nitrogenase activity in photosynthetic organisms

Research Project

Project/Area Number 24770040
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

NOMATA Jiro  東京工業大学, 資源化学研究所, 助教 (40583216)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsレドックス / 金属中心 / シアノバクテリア / 窒素固定 / プロテオミクス / 嫌気
Outline of Final Research Achievements

Anabaena sp. strain PCC 7120 (A.7120) is a diazotrophic cyanobacterium, which can fix atmospheric nitrogen by utilizing the solar energy. Thioredoxin (Trx) is a class of small redox proteins known to play a role in many important biological processes. Recently, NifU protein was captured in the A.7120 by using the proteomics-method named “Thioredoxin affinity chromatography” to comprehensively isolate TrxM target proteins. NifU protein plays a crucial role as a scaffold protein for the assembly of the Fe-S clusters required for the full activation of Fe-protein and MoFe-protein of nitrogenase. In this work, based on the biochemical analysis, we showed disulfide bond(s) formed in the N-terminal catalytic domain in NifU protein was reduced by Trx M. Further, we observed that the scaffold activity of the catalytic domain of NifU is enhanced in the presence of Trx-system, suggesting that TrxM is involved in the Fe-S cluster biogenesis by NifU.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitmgenase-like enzyme, from Rhodobacter capsulatus2013

    • Author(s)
      Nomata J., Kondo T., Itoh S., Fujita Y.
    • Journal Title

      FEBS Letters

      Volume: 587 Issue: 18 Pages: 3142-3147

    • DOI

      10.1016/j.febslet.2013.07.054

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light2013

    • Author(s)
      Yusuke Tsukatani, Haruki Yamamoto, Jiro Harada, Taichi Yoshitomi, Jiro Nomata, Masahiro Kasahara, Tadashi Mizoguchi, Yuichi Fujita, and Hitoshi Tamiaki
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 1217-1217

    • DOI

      10.1038/srep01217

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Anabaena sp. strain PCC 7120の鉄硫黄クラスター生合成蛋白質はTrxと相互作用する2015

    • Author(s)
      野亦次郎, 前田真希, 井須敦子, 井上和仁, 久堀徹
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Anabaena sp. strain PCC 7120におけるNifU蛋白質によるFeSクラスター形成はTrxに依存する2014

    • Author(s)
      野亦次郎、前田真希、井須敦子、井上和仁、久堀徹
    • Organizer
      日本植物生理学会
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
  • [Presentation] 窒素固定性シアノバクテリアAnabaena sp. PCC7120におけるチオレドキシン標的タンパク質の網羅的探索2013

    • Author(s)
      野亦次郎、前田真希、井上和仁、久堀徹
    • Organizer
      光合成学会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
  • [Presentation] Redox regulation of Iron-Sulfer clusters assembly on NifU scaffold protein from Anabaena sp.strain PCC 71202013

    • Author(s)
      Jiro Nomata, Atsuko Isu, Toru Hisabori
    • Organizer
      International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎
    • Related Report
      2013 Research-status Report
  • [Presentation] 窒素固定性シアノバクテリアAnabaena sp. PCC7120のチオレドキシン破壊株を用いたレドックス制御機構の解析2013

    • Author(s)
      土屋昭洋、野亦次郎、久堀徹
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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