Dual, realtime minitoring of Ca2+ and ROS signals in plant cells

• Project/Area Number: 24770041
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Gifu Women's University (2013); Nagoya University (2012)
• Principal Investigator: FURUICHI Takuya 岐阜女子大学, 家政学部, 准教授 (80436998)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 細胞内シグナル伝達 / 活性酸素種 / ROS / 生物発光 / リアルタイム計測 / カルシウムシグナル / ROSシグナル

Research Abstract

Reactive oxygen species (ROS) play key roles in intra- / inter-cellular signal transduction. Recent studies revealed that Ca2+-signal and ROS-signal are interplaying for the precise intracellular signal transduction through generating [Ca2+]c oscillation and playing the other key roles of still unknown. In the present study, we tried to develop a novel method to monitor [ROS]c and [Ca2+]c simultaneously using Ca2+- or ROS-sensitive photoproteins and spectrometry of luminescence to clarify the physiological roles and mechanisms of ROS-signal and their interplay with Ca2+-signal in plants.

Research Products

• [Journal Article] Mechanosensitive channels are activated by stress in the actin stress fibers, which could be involved in gravity sensing in plants (2014)
  • Author(s): Tatsumi, H., Furuichi, T., Nakano, M., Toyota, M., Hayakawa, K., Sokabe, M. and Iida, H.
  • Journal Title: Plant Biol. Volume: 16 Pages: 18-22
• [Journal Article] New candidates for mechanosensitive channels potentially involved in gravity sensing in Arabidopsis (2014)
  • Author(s): Iida, H., Furuichi, T., Nakano, M., Toyota, M., Sokabe, M. and Tatsumi, H.
  • Journal Title: Plant Biol. Volume: 16 Pages: 39-42
• [Journal Article] Calcium Mobilizations in Response to Changes in the Gravity Vector in Arabidopsis Seedlings : Possible Cellular Mechanisms (2014)
  • Author(s): Tatsumi, H., Toyota, M., Furuichi, T., and Sokabe, M.
  • Journal Title: Plant Signal. Behav. Volume: (In Press)
• [Journal Article] New candidates for mechano-sensitive channels potentially (2014)
  • Author(s): H. Iida, T. Furuichi, M. Nakano, M. Toyota, M. Sokabe & H. Tatsumi
  • Journal Title: Plant Biology Volume: 16(Suppl. 1) Issue: s1 Pages: 39-42
  • DOI: 10.1111/plb.12044
  • Peer Reviewed
• [Journal Article] Calcium Mobilizations in Response to Changes in the Gravity Vector in Arabidopsis Seedlings: Possible Cellular Mechanisms (2014)
  • Author(s): Tatsumi, H., Toyota, M., Furuichi, T., and Sokabe, M.
  • Journal Title: Plant Signal. Behav. Volume: 9 Issue: 8 Pages: e29099-e29099
  • DOI: 10.4161/psb.29099
  • Peer Reviewed
• [Journal Article] Analysis of a gravistimulation-specific Ca^2+ signature in Arabidopsis using parabolic flights (2013)
  • Author(s): Toyota, M., Furuichi, T., Sokabe, M., and Tatsumi, H.
  • Journal Title: Plant Physiol. Volume: 163 Pages: 543-554
• [Journal Article] Analyses of a gravistimulation-specific Ca2+ signature in Arabidopsis using parabolic flights (2013)
  • Author(s): Masatsugu Toyotaa, Takuya Furuichi, Masahiro Sokabea, and Hitoshi Tatsumi
  • Journal Title: Plant Physiology Volume: 163 Issue: 2 Pages: 543-554
  • DOI: 10.1104/pp.113.223313
  • Peer Reviewed / Open Access
• [Journal Article] A Microfluidic Device to Reduce Treatment Time of Intracytoplasmic Sperm Injection (2013)
  • Author(s): Koji Matsuura, Takuya Uozumi, Takuya Furuichi, Ikuyo Sugimoto, Mieko Kodama, Hiroaki Funahashi
  • Journal Title: Fertility and Sterility Volume: 99 Issue: 2 Pages: 400-407
  • DOI: 10.1016/j.fertnstert.2012.10.022
  • Peer Reviewed
• [Journal Article] Chloroplast-mediated activation of plant immune signalling in Arabidopsis. (2012)
  • Author(s): Nomura H, Komori T, Uemura S, Kanda Y, Shimotani K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika IN, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T.
  • Journal Title: Nat Commun.26 Volume: 3 Issue: 1 Pages: 926-926
  • DOI: 10.1038/ncomms1926
  • Peer Reviewed
• [Journal Article] Expression of Arabidopsis MCA1 enhanced mechanosensitive channel activity in the Xenopus laevis oocyte plasma membrane (2012)
  • Author(s): Furuichi, T., Iida, H., Sokabe, M. and Tatsumi, H.
  • Journal Title: Plant Sig. Behav. Volume: 7(8) Pages: 1022-1026
• [Journal Article] Expression of Arabidopsis MCA1 enhanced mechanosensitive channel activity in the Xenopus laevis oocyte plasma membrane (2012)
  • Author(s): Takuya Furuichi, Hidetoshi Iida, Masahiro Sokabe and Hitoshi Tatsumi
  • Journal Title: Plant Signaling and Behavior 7 Volume: 7 Issue: 8 Pages: 1022-1026
  • DOI: 10.4161/psb.20783
  • Peer Reviewed
• [Presentation] Use of a photoprotein from shellfish for the real-time monitoring of cytoplasmic ROS level (2013)
  • Author(s): Furuichi, T. and Kuse, M.
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] Mechanosensitive channels are activated via actin stress fibers, which may be involved in gravity sensing in plants (2012)
  • Author(s): Tatsumi, H., Furuichi^, T., Nakano^, M., Toyota^, M., Hayakawa^, K., Sokabe^, M. and Iida, H.
  • Organizer: ISLSWG Workshop„Plant Biology in Space"–Satellite Meeting to the Plant Biology Congress 2012–
  • Place of Presentation: Freiburg, Germany
• [Presentation] New candidate for the mechanosensitive channel that may be involved in gravity sensing (2012)
  • Author(s): Iida, H., Furuichi, T., Nakano, M., Toyota, M., Sokabe, M. and Tatsumi, H.
  • Organizer: ISLSWG Workshop„Plant Biology in Space"–Satellite Meeting to the Plant Biology Congress 2012–
  • Place of Presentation: Freiburg, Germany
• [Presentation] Roles and mechanisms of calcium signaling in plant environmental responses (2012)
  • Author(s): Furuichi, T
  • Organizer: International Conference on Advances in Plant Sciences (Plants2012)
  • Place of Presentation: Chiang Mai, Thailand
• [Presentation] Real-time monitoring of the intracellular ROS signal using a novel photoprotein (2012)
  • Author(s): Furuichi, T
  • Organizer: The 4th NIBB-MPIPZ-TIL Symposium
  • Place of Presentation: Okazaki, Japan
• [Presentation] Mechanosensitive channels are activated via actin stress fibers, which may be involved in gravity sensing in plants (2012)
  • Author(s): Tatsumi, H., Furuichi, T., Nakano, M., Toyota, M., Hayakawa, K., Sokabe, M. and Iida, H.
  • Organizer: ISLSWG Workshop; Plant Biology in Space (Satellite Meeting to the Plant Biology Congress 2012)
  • Place of Presentation: フライブルグ（ドイツ）
• [Presentation] New candidate for the mechanosensitive channel that may be involved in gravity sensing (2012)
  • Author(s): Iida, H., Furuichi, T., Nakano, M., Toyota, M., Sokabe, M. and Tatsumi, H.
  • Organizer: ISLSWG Workshop; Plant Biology in Space (Satellite Meeting to the Plant Biology Congress 2012)
  • Place of Presentation: フライブルグ（ドイツ）
• [Presentation] Roles and mechanisms of calcium signaling in plant environmental responses (2012)
  • Author(s): Furuichi, T.
  • Organizer: International Conference on Advances in Plant Sciences (Plants2012)
  • Place of Presentation: チェンマイ（タイ）
• [Presentation] Use of a photoprotein from shellfish for the real-time monitoring of cytoplasmic ROS level
  • Author(s): Furuichi, T. and Kuse, M.
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山