Analysis of symbiotic signaling networks of arbuscular mycorrhiza and root nodule symbiosis.

• Project/Area Number: 24770050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TAKEDA Naoya 基礎生物学研究所, 共生システム研究部門, 助教 (60571081)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 植物微生物相互作用 / 共生 / アーバスキュラー菌根共生 / 根粒共生 / ミヤコグサ / トランスクリプトーム解析 / トランスクリプトーム / 遺伝子制御ネットワーク

Research Abstract

Arbuscular mycorrhiza (AM) is a plant-fungal interaction that confers great advantages for the host plant growth. Here we investigated AM phenotypes of AM mutants cerberus and nsp1 and revealed novel regulation mechanisms of AM fungal infection. Transcriptome analysis of these mutants indicated that gibberellin (GA) signaling affects AM fungal infection in the host root. Detailed analysis of GA effect on AM revealed that GA signaling integrated into symbiotic signaling and regulates symbiosis gene expression. We also analyzed RAMI1 that had been isolated as a transcriptional regulator that bind to AM specific cis element. Analysis of RAMI1 indicated that RAMI1 has important roles not only in AM but also in root nodule symbiosis and non-symbiotic physiological functions.

Research Products

• [Journal Article] Common symbiosis genes CERBERUS and NSP1 provide additional insight into the establishment of arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus. (2014)
  • Author(s): Nagae M, Takeda N, Kawaguchi M
  • Journal Title: Plant Signaling & Behavior Volume: 9
  • Peer Reviewed
• [Journal Article] CERBERUS and NSP1 of Lotus japonicus are common symbiosis genes that modulate arbuscular mycorrhiza development (2013)
  • Author(s): Takeda N, Tsuzuki S, Suzaki T, Kawaguchi M
  • Journal Title: Plant Cell Physiology Volume: Vol54 (featured on the Research Highlights) Pages: 1711-23
  • NAID: 40019838872
  • Peer Reviewed
• [Journal Article] TOO MUCH LOVE, a novel kelch repeat-containing F-box protein, functions in the long-distance regulation of the legume-Rhizobium symmbiosis (2013)
  • Author(s): Takahara M, Magori S, Soyano T, Okamoto S, Yoshida C, Yano K, Sato S, Tabata S, Yamaguchi K, Shigenobu S, Takeda N, Suzaki T, Kawaguchi M
  • Journal Title: Plant Cell Physiology Volume: Vol54 Pages: 433-47
  • Peer Reviewed
• [Journal Article] TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus (2013)
  • Author(s): Suzaki T, Kim, C.S, Takeda N, Szczyglowski, K. and Kawaguchi, M
  • Journal Title: Development Volume: Vol.140 Pages: 353-361
  • Peer Reviewed
• [Journal Article] CERBERUS and NSP1 of Lotus japonicus are common symbiosis genes that modulate arbuscular mycorrhiza development. (2013)
  • Author(s): Takeda, N., Tsuzuki, S., Suzaki, T., Parniske, M., and Kawaguchi, M.
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 10 Pages: 1711-1723
  • DOI: 10.1093/pcp/pct114
  • NAID: 40019838872
  • Peer Reviewed
• [Journal Article] Too much love, a novel Kelch repeat-containing F-box protein, functions in the long-distance regulation of the legume-Rhizobium symbiosis (2013)
  • Author(s): Takahara, M., Magori, S., Soyano, T., Okamoto, S., Yoshida, C., Yano, K., Sato, S., Tabata, S., Yamaguchi, K., Shigenobu, S., et al
  • Journal Title: Plant Cell Physiol Volume: 54 Issue: 4 Pages: 433-447
  • DOI: 10.1093/pcp/pct022
  • NAID: 10031163838
  • Peer Reviewed
• [Journal Article] TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus. (2013)
  • Author(s): Suzaki, T, Kim, C.S, Takeda, N, Szczyglowski, K. and Kawaguchi, M.
  • Journal Title: Development Volume: 140 Issue: 2 Pages: 353-361
  • DOI: 10.1242/dev.089631
  • Peer Reviewed
• [Journal Article] Common symbiosis genes CERBERUS and NSP1 provide additional insight into the establishment of arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus
  • Author(s): Nagae M, Takeda N, Kawaguchi M
  • Journal Title: Plant Signaling & Behavior Volume: (in press)
  • Peer Reviewed
• [Journal Article] Endoreduplication-mediated initiation of symbiotic organ development in Lotus japonicus
  • Author(s): Suzaki T, Ito M, Yoro E, Sato S, Harakawa H, Takeda N, Kawaguchi M
  • Journal Title: Development Volume: (in press)
  • Peer Reviewed
• [Presentation] ジベレリンシグナルが菌根共生・根粒共生に及ぼす影響 (2014)
  • Author(s): 武田直也, 他
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
  • Year and Date: 2014-03-18
• [Presentation] ジベレリンシグナルが菌根共生・根粒共生に及ぼす影響 (2014)
  • Author(s): 武田直也
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山県 富山大学
• [Presentation] Gibberellin signaling interferes with symbiosis signaling pathway, which alters symbiotic gene expression and fungal colonization during arbuscular mycorrhiza (2013)
  • Author(s): Takeda N, et al.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 2013-10-14
• [Presentation] 菌根菌・根粒菌感染に及ぼすジベレリンシグナルの役割 (2013)
  • Author(s): 武田直也., 他
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-21
• [Presentation] Gibberellin signaling interferes with symbiosis signaling pathway, which alters symbiotic gene expression and fungal colonization during arbuscular mycorrhiza (2013)
  • Author(s): Takeda N.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎県 シーガイア
• [Presentation] Roles of Gibberellin signaling in arbuscular mycorrhiza and root nodule symbiosis. (2013)
  • Author(s): 武田 直也
  • Organizer: 植物生理学会年会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] Common Symbiosis System reveals infection mechanism of Arbuscular Mycorrhizal fungi in leguminous plant Lotus japonicus (2012)
  • Author(s): Takeda N
  • Organizer: 1st Molecular Mycorrhiza Meeting
  • Place of Presentation: ドイツ
  • Year and Date: 2012-09-06
  • Invited
• [Presentation] COMMON SYMBIOSIS SYSTEM REVEALS INFECTION MECHANISM OF ARBUSCULAR MYCORRHIZAL FUNGI IN LEGUMINOUS PLANT Lotus japonicus (2012)
  • Author(s): 武田 直也
  • Organizer: 1st Molecular Mycorrhizal meeting (招待講演)
  • Place of Presentation: ドイツ ミュンヘン大学
  • Invited
• [Presentation] 新たな共通共生因子の共生体感染過程における役割 (2012)
  • Author(s): 武田 直也
  • Organizer: 植物微生物研究交流会
  • Place of Presentation: 神戸大学（兵庫県）