Elucidation of RNA metabolism regulation important for plant cell totipotency

• Project/Area Number: 24770052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology (2014); Institute of Physical and Chemical Research (2012-2013)
• Principal Investigator: OHTANI Misato 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60435633)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分化全能性 / RNA代謝 / プレｍRNAスプライシング / RNA品質管理 / snRNA / シュート再生 / 脱分化 / 器官再生 / pre-mRNAスプライシング / 国際情報交換

Outline of Final Research Achievements

The cell potentiality to give rise to a whole body is called “totipotency”, and plant somatic cells are considered to retain this ability. In this project, molecular genetics works were performed with Arabidopsis thaliana mutants, to reveal molecular mechanisms underlying plant cell totipotency, especially from the viewpoint of RNA metabolism regulation. The obtained results suggested that specific RNA metabolism regulatory pathways, such as pre-mRNA splicing regulation and/or RNA quality control mechanism, have critical roles in plant cell totipotency.

Research Products

• [Journal Article] Cell dedifferentiation and organogenesis in vitro require more snRNA than does seedling development in Arabidopsis thaliana. (2015)
  • Author(s): Ohtani M, Takebayashi A, Hiroyama R, Xu B, Kudo T, Sakakibara H, Sugiyama M, Demura T
  • Journal Title: Journal of Plant Research Volume: 128 Issue: 3 Pages: 371-380
  • DOI: 10.1007/s10265-015-0704-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins (2015)
  • Author(s): Yamaguchi M, Nagahage ISP, Ohtani M, Ishikawa T, Uchimiya H, Kawai-Yamada M, Demura T
  • Journal Title: Plant Biotechnology Volume: 32 Issue: 2 Pages: 119-123
  • DOI: 10.5511/plantbiotechnology.15.0208a
  • NAID: 130005085996
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of RNA metabolism is important for in vitro dedifferentiation of plant cells. (2015)
  • Author(s): Ohtani M
  • Journal Title: Journal of Plant Research Volume: 128 Issue: 3 Pages: 361-369
  • DOI: 10.1007/s10265-015-0700-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants. (2015)
  • Author(s): Nakano Y, Yamaguchi M, Endo H, Rejab NA, Ohtani M
  • Journal Title: Frontiers in Plant Science Volume: 6 Pages: 288-288
  • DOI: 10.3389/fpls.2015.00288
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation (2015)
  • Author(s): Endo H, Yamaguchi M, Tamura T, Nakano Y, Nishikubo N, Yoneda A, Kato K, Kubo M, Kajita S, Katayama Y, Ohtani M, Demura T
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 2 Pages: 242-254
  • DOI: 10.1093/pcp/pcu134
  • Peer Reviewed / Open Access
• [Journal Article] Local gene silencing in plants via synthetic dsRNA and carrier peptide (2014)
  • Author(s): Numata, K., Yoshizumi, T., Ohtani, M., Demura, T., Kodama, Y.
  • Journal Title: Plant Biotechnology Journal Volume: 印刷中
  • Peer Reviewed
• [Journal Article] A poly (A)-specific ribonuclease directly regulates the poly (A) status of mitochondrial mRNA in Arabidopsis. (2013)
  • Author(s): Hirayama,T.,et al.
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 2247-2247
  • DOI: 10.1038/ncomms3247
  • Peer Reviewed
• [Journal Article] Arabidopsis RID1, a DEAH-box RNA helicase involved in pre-mRNA splicing, plays an essential role in plant development (2013)
  • Author(s): Ohtani, M., Demura, T. and Sugiyama, M.
  • Journal Title: Plant Cell Volume: -
  • Peer Reviewed
• [Presentation] Roles of snRNA cap hypermethylation in plant development (2015)
  • Author(s): Hiroyama R, Demura T, Ohtani M
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物における発生段階依存的な小分子RNA産生活性の解析 (2015)
  • Author(s): 田原緑、大谷美沙都、金勝一樹、森山裕充、福原敏行
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物発生におけるsnRNAキャップトリメチル化の役割 (2015)
  • Author(s): 大谷美沙都
  • Organizer: 第4回植物RNA研究者ネットワークシンポジウム
  • Place of Presentation: 京都大学（京都府京都市）
  • Year and Date: 2015-01-19 – 2015-01-20
  • Invited
• [Presentation] Cell dedifferentiation and organogenesis in vitro require more snRNA than does seedling development in Arabidopsis thaliana. (2014)
  • Author(s): Ohtani M, Takebayashi A, Hiroyama R, Xu B, Kudo T, Sakakibara H, Sugiyama M, Demura T
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Critical roles of snRNP biogenesis for spliceosome formation in plant regeneration (2014)
  • Author(s): Ohtani M
  • Organizer: Post-transcriptional Gene Expression Regulation in Plants
  • Place of Presentation: Poznan (Poland)
  • Year and Date: 2014-06-30 – 2014-07-02
• [Presentation] 植物細胞の分化全能性を支えるpre-mRNAスプライシング制御 (2014)
  • Author(s): 大谷美沙都、出村拓、杉山宗隆
  • Organizer: 日本植物生理学会第55回年会
  • Place of Presentation: 富山大学（富山）
  • Invited
• [Presentation] 植物におけるsnRNA生合成制御とその生理的意義 (2013)
  • Author(s): 大谷美沙都
  • Organizer: RNAから植物を考える~植物をささえるRNA機能~（第３回植物研究者ネットワークシンポジウム）、
  • Place of Presentation: 北海道大学（札幌）
  • Invited
• [Presentation] 植物におけるsnRNAキャップ修飾の生理的意義 (2013)
  • Author(s): 廣山涼子、出村拓、大谷美沙都
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 県民文化会館（愛媛、松山）
• [Presentation] Critical roles of RID1 DEAH-box RNA helicase functioning in pre-mRNA splicing for organogenesis in vitro and in planta (2012)
  • Author(s): Ohtani, M., Demura, T. and Sugiyama, M.
  • Organizer: 23th International Conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
• [Presentation] Physiological roles of snRNA hypermethylation in plant: lessons from Arabidopsis TGS genes (2012)
  • Author(s): Hiroyama, R., Demura, T. and Ohtani, M.
  • Organizer: Plant RNA Meeting
  • Place of Presentation: Vienna, Austria
• [Presentation] Essential requirement for Arabidopsis RID1, a DEAH-box RNA helicase involved in pre-mRNA splicing, in plant development (2012)
  • Author(s): Ohtani, M.
  • Organizer: Frontiers in Plant RNA Research 2012
  • Place of Presentation: Sapporo, Japan
  • Invited
• [Presentation] 植物細胞の分化全能性を支えるpre-mRNAスプライシング制御
  • Author(s): 大谷美沙都
  • Organizer: RNA worldへの階梯（東京理科大学RNA科学総合研究センター公開シンポジウム）
  • Place of Presentation: 東京理科大学（東京）
  • Invited
• [Remarks] 植物RNA研究ネットワーク
  • URL: https://www.sci.hokudai.ac.jp/~yukako/RNA/
• [Remarks] 理研プレスリリースページ
  • URL: http://www.riken.jp/pr/press/2013/20130615_1/
• [Remarks] 岡山大プレスリリースページ
  • URL: http://www.okayama-u.ac.jp/tp/release/release_id86.html
• [Remarks] 植物RNA研究ネットワーク
  • URL: https://www.sci.hokudai.ac.jp/~yukako/RNA/
• [Remarks] Frontiers in Plant RNA Research 2012
  • URL: http://www.riken.jp/bmep/teams/cellulose_production/test/index.html
• [Patent(Industrial Property Rights)] カルス誘導剤及びカルス誘導方法 (2014)
  • Inventor(s): 中野雄司、浅見忠男、山上あゆみ、長田裕之、大谷美沙都、出村拓
  • Industrial Property Rights Holder: 中野雄司、浅見忠男、山上あゆみ、長田裕之、大谷美沙都、出村拓
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-117832
  • Filing Date: 2014-06-06