| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
This study aimed to analyze gamete interaction during double fertilization specific to flowering plants using molecular cell biological strategies. Especially, this study focused on elucidating the molecular functions of transmembrane proteins identified in male gametes. When a proteomics analysis of Lilium generative cell membrane was performed based on the RNA sequencing data, a novel transmembrane protein named LGM1 was newly detected. LGM1 proved to be a small molecule, which possesses four transmembrane domains and conserved in algae, moss and lycophytes. As a result of molecular cytological analyses of LGM1, it was found that LGM1 is expressed specifically in male gametes.
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