Ca2+ imaging analysis of neural circuitry that supports olfactory memory formation in Drosophila
| Project/Area Number |
24770066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIROI Makoto 東京大学, 分子細胞生物学研究所, 助教 (80597831)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ショウジョウバエ / 匂い記憶 / Caイメージング / 記憶痕跡 / 学習・記憶 / 神経生物学 / 記憶・学習 / 学習 / 記憶 / イメージング / 可塑性 / 神経回路 |
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| Research Abstract |
To identify neural circuitry that support the memory formation in Drosophila, I analyzed Ca imaging from the brain during olfactory conditioning. I first established a functional imaging system to monitor neuronal activities in the brain of awake, behaving animals responding to sensory stimuli. The in vivo preparation to be established here should provide a consistent way of analyzing gene function during olfactory learning and memory. The functional imaging allows us to analyze temporal and spatial dynamics of many neurons simultaneously. This is an important feature because populations of the ~2500 intrinsic mushroom body neurons will be largely heterogeneous in function and fate after training. In addition, I adopted principal component analysis on the data that provides the opportunity for more comprehensive and detailed analyses of cell physiology that will expand our understanding of memory storage.
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Report
(3 results)
Research Products
(12 results)
