Molecular mechanism regulating the alternation of generations in land plants using the transcription factor

• Project/Area Number: 24770077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: The University of Tokyo (2013-2014); Hiroshima University (2012)
• Principal Investigator: SAKAKIBARA Keiko 東京大学, 理学(系)研究科(研究院), 助教 (90590000)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 陸上植物 / 世代交代 / アポスポリー / 転写因子 / ホメオボックス遺伝子 / ヒメツリガネゴケ / 次世代シーケンサー / KNOX

Outline of Final Research Achievements

Land plants undergo an alternation of generations, producing multicellular bodies in both 1n and 2n generations. We found the transcription factor KNOX2 function in the repression of haploid developmental program in the diploid body. To identify the molecular mechanism regulating the alternation of generations by KNOX2 genes, we performed the comparative transcriptome analysis using the early developing embryos of wild-type and KNOX2 mutant in a moss Physcomitrella patens.
As the result of comparative transcriprome analysis, we found that the expression level of several transcription factors, secondary metabolic synthetic genes was altered in the KNOX2 mutant embryo. We hypothesize that the control of gene regulatory network and metabolism is important to regulate the alternations of generations in land plants.

Research Products

• [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. (2015)
  • Author(s): Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
  • Journal Title: Development Volume: 142 Issue: 3 Pages: 444-453
  • DOI: 10.1242/dev.113167
  • Peer Reviewed / Open Access
• [Journal Article] Antagonistic Roles for KNOX1 and KNOX2 Genes in Patterning the Land Plant Body Plan Following an Ancient Gene Duplication (2015)
  • Author(s): Furumizu, C., Alvarez, J. P., Sakakibara, K., Bowman J. L.
  • Journal Title: PLOS Genetics Volume: 11 Issue: 2 Pages: e1004980-e1004980
  • DOI: 10.1371/journal.pgen.1004980
  • Peer Reviewed / Open Access
• [Journal Article] 陸上植物の複相の巨大化と複雑化に貢献したホメオボックス遺伝子の重複と機能分化 (2014)
  • Author(s): 榊原恵子
  • Journal Title: 細胞工学 Volume: 33 Pages: 872-877
  • NAID: 40020162174
• [Journal Article] Evolution of the class IV HD-zip gene family in streptophytes. (2013)
  • Author(s): Christopher S. Zalewski, Sandra K. Floyd, Chihiro Furumizu, Keiko Sakakibara, Dennis W. Stevenson, John L. Bowman
  • Journal Title: Molecular Biology and Evolution Volume: 30 Issue: 10 Pages: 2347-2365
  • DOI: 10.1093/molbev/mst132
  • Peer Reviewed
• [Journal Article] 陸上植物の世代交代の謎にせまる～遺伝子重複と機能分化による胞子体の劇的な形態進化～ (2013)
  • Author(s): 榊原恵子
  • Journal Title: 化学と生物 Volume: 51 Pages: 595-596
  • NAID: 10031195844
• [Journal Article] 蘚苔類の世代交代とその制御因子 (2013)
  • Author(s): 榊原恵子
  • Journal Title: 蘚苔類研究 Volume: 10 Pages: 393-397
  • NAID: 110009687935
  • Peer Reviewed
• [Journal Article] 「コケ植物のエボデボから見えてきた胞子体の複雑化」 あんな形，こんなできかた―エボデボ研究最前線［植物篇］ (2013)
  • Author(s): 榊原恵子
  • Journal Title: 生物の科学遺伝 Volume: 1 Pages: 39-44
• [Journal Article] KNOX2 genes regulate the haploid-to-diploid morphological transition in land plants (2013)
  • Author(s): Sakakibara, K., Ando, S., Yip, H.K., Tamada, Y., Hiwatashi, Y., Murata, T., Deguchi, H., Hasebe, M., and Bowman, J.L.
  • Journal Title: Science Volume: 339 Issue: 6123 Pages: 1067-1070
  • DOI: 10.1126/science.1230082
  • Peer Reviewed
• [Journal Article] KNOX2遺伝子は陸上植物の世代交代において複相から単相への移行を抑制する (2013)
  • Author(s): 榊原恵子 John L. Bowman
  • Journal Title: ライフサイエンス 新着論文レビュー Volume: なし
• [Presentation] Evolution of the alternation of generation in Land Plants (2015)
  • Author(s): Sakakibara, K.
  • Organizer: Botany 2015
  • Place of Presentation: The Shaw Conference Centre (Edmonton, Alberta, Canada)
  • Year and Date: 2015-07-25 – 2015-07-29
  • Invited
• [Presentation] Identification of downstream genes of KNOX2 transcription factor that regulates alternations of generations in land plants (2015)
  • Author(s): 榊原恵子、西山智明、塚谷裕一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学 (東京都世田谷区)
  • Year and Date: 2015-03-16 – 2016-03-18
• [Presentation] KNOX遺伝子族の進化による陸上植物複相の複雑化 (2014)
  • Author(s): 榊原恵子、古水千尋、Bowman, J. L.
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学 (神奈川県川崎市)
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] コケ植物の再生能力とその制御因子 (2014)
  • Author(s): 榊原恵子
  • Organizer: 日本蘚苔類学会
  • Place of Presentation: 奥入瀬渓流ホテル (青森県十和田市)
  • Year and Date: 2014-08-26 – 2014-08-28
• [Presentation] KNOX遺伝子は陸上植物の世代交代を制御する (2014)
  • Author(s): 榊原恵子
  • Organizer: 進化学会
  • Place of Presentation: 高槻現代劇場 (大阪府高槻市)
  • Year and Date: 2014-08-20 – 2014-08-23
• [Presentation] WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens (2014)
  • Author(s): Sakakibara, K., Reisewitz, P., Aoyama, T., Friedrich, T., Ando, S., Sato, Y., Nishiyama, T., Tamada, Y., Hiwatashi, Y., Kurata, T., Ishikawa, M., Deguchi, H., Rensing, S. A., Werr, W., Murata, T., Hasebe, M., Laux, T.
  • Organizer: 日本発生生物学会第47回年会
  • Place of Presentation: Winc Aichi (Nagoya, Aichi)
  • Year and Date: 2014-05-28 – 2014-05-30
• [Presentation] KNOX２遺伝子は陸上植物の世代交代を制御する (2013)
  • Author(s): 榊原恵子 安藤沙友里 Hoichong Karen Yip 玉田洋介 日渡祐二 村田隆 出口博則 長谷部光泰 John L. Bowman
  • Organizer: 蘚苔類学会
  • Place of Presentation: 岡山理科大
• [Presentation] 陸上植物の世代交代を制御するKNOX2遺伝子のターゲット探索 (2013)
  • Author(s): 榊原恵子
  • Organizer: 次世代シーケンサー現場の会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ヒメツリガネゴケをモデルとして発生進化学的解析による陸上植物の異形世代交代を司る分子機構の解明 (2013)
  • Author(s): 榊原恵子
  • Organizer: 植物学会
  • Place of Presentation: 北海道大学
  • Invited
• [Presentation] Physcomitrella WOX genes are necessary for the formation of stem cells from differentiated leaf cells (2012)
  • Author(s): Sakakibara, K., Aoyama, T., Ando, S., Sato, Y., Kurata, T., Murata, T., Nishiyama, T., Hasebe, M.
  • Organizer: Moss 2012
  • Place of Presentation: New York, USA
• [Presentation] ヒメツリガネゴケからみる陸上植物の発生進化 (2012)
  • Author(s): 榊原恵子
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
• [Presentation] Physcomitrella WOX genes are necessary for the formation of stem cells from differentiated leaf cells (2012)
  • Author(s): Sakakibara, K., Aoyama, T., Ando, S., Sato, Y., Sugimoto, N., Ohshima, M., Murata, T., Nishiyama, T., Hasebe, M.
  • Organizer: 第３回Marchantia workshop
  • Place of Presentation: 阿蘇、熊本
  • Invited
• [Presentation] Physcomitrella WOX genes are necessary for the formation of stem cells from differentiated leaf cells (2012)
  • Author(s): Sakakibara, K., Aoyama, T., Ando, S., Sato, Y., Sugimoto, N., Ohshima, M., Murata, T., Nishiyama, T., Hasebe, M.
  • Organizer: The 4th NIBB-MPIPZ-TLL Symposium Arabidopsis and Emerging Model Systems
  • Place of Presentation: 愛知、岡崎
• [Presentation] KNOX遺伝子は陸上植物の世代交代を制御する
  • Author(s): 榊原恵子
  • Organizer: 東京大学生命科学セミナー
  • Place of Presentation: 東京大学
• [Remarks] ［研究成果］「サイエンス」誌に発表！陸上植物の進化の謎、世代交代成立の仕組みを解明
  • URL: http://www.hiroshima-u.ac.jp/news/show/id/16351
• [Remarks] 160年来の謎、陸上植物の世代交代を制御する因子の発見
  • URL: http://www.nibb.ac.jp/press/2013/03/01.html
• [Remarks] KNOX2遺伝子は陸上植物の世代交代において複相から単相への移行を抑制する
  • URL: http://first.lifesciencedb.jp/archives/6644