Mitochondrial genomics of flatfishes: Comprehensive molecular phylogeny and genome structural evolution

• Project/Area Number: 24770082
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: National Museum of Nature and Science, Tokyo
• Principal Investigator: SATOH Takashi 独立行政法人国立科学博物館, 標本資料センター, 特定非常勤研究員 (60436516)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: カレイ目 / ミトコンドリアゲノム / 分子系統 / 分子進化 / 遺伝子配置 / 系統解析

Outline of Final Research Achievements

Using whole mitochondrial genome sequences, we aimed at construction of a robust phylogenetic tree which covered all major lineages of flatfishes. Finally, new data of 100 flatfishes were determined, and a comprehensive phylogenetic tree on a total of 180 species including outgroups and existing data was constructed. As a result, monophyly of the flatfish was supported with a weak value, and monophyly of 2 suborders and 3 superfamilies which accorded with the latest classification system was also supported. The Paralichthidae (Large-tooth flounders) was clealy shown to be paraphyletic group divided into two groups. Furthermore, 8 patterns of gene rearrangements were found in 4 families of flatfishes.

Research Products

• [Journal Article] Mitochondrial genomic investigation of flatfish monophyly. (2014)
  • Author(s): Campbell M. A., J. A. Lopez, T. P. Satoh, W. J. Chen and M. Miya
  • Journal Title: Gene Volume: 551(2) Issue: 2 Pages: 176-182
  • DOI: 10.1016/j.gene.2014.08.053
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] First Record of the Longlure Flatfish, Asterorhombus filifer (Pleuronectiformes: Bothidae), from Japan (2014)
  • Author(s): Katayama, E., T. P. Satoh and K. Matsuura.
  • Journal Title: Species Diversity Volume: 19 Issue: 2 Pages: 85-89
  • DOI: 10.12782/sd.19.2.085
  • NAID: 110009872232
  • ISSN: 1342-1670, 2189-7301
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mitogenomic circumscription of a novel percomorph fish clade mainly comprising "Syngnathoidei" (Teleostei) (2014)
  • Author(s): Ha Yeun Song, K. Mabuchi, T. P. Satoh, J. A. Moore, Y. Yamanoue, M. Miya, M. Nishida
  • Journal Title: Gene Volume: 542 Issue: 2 Pages: 146-155
  • DOI: 10.1016/j.gene.2014.03.040
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Scombridae (tunas and mackerels) : members of a Paleogene adaptive radiation with 14 other pelagic fish families (2013)
  • Author(s): Miya, M., Friedman, M., Satoh, T.P., Takeshima, H., Sado, T., Iwasaki, W., Yamanoue, Y., Nakatani, M., Mabuchi, K., Inoue, J.G., Poulsen, J.Y., Fukunaga, T., Sato, Y. and Nishida, M
  • Journal Title: PLOS ONE Volume: 8 Issue: 9 Pages: e73535-e73535
  • DOI: 10.1371/journal.pone.0073535
  • Peer Reviewed
• [Journal Article] MitoFish and MitoAnnotator : a mitochondrial genome database of fish with an accurate and automatic annotation pipeline (2013)
  • Author(s): Iwasaki, W., Fukunaga T., Isagozawa R., Yamada K., Maeda Y., Satoh T.P., Sado T., Mabuchi K., Takeshima H., Miya, M. and Nishida M
  • Journal Title: Molecular Biology and Evolution Volume: 30 Issue: 11 Pages: 2531-2540
  • DOI: 10.1093/molbev/mst141
  • Peer Reviewed
• [Presentation] ミトコンドリアゲノム全長配列によるカレイ目魚類の網羅的系統解析 (2014)
  • Author(s): 佐藤 崇・宮 正樹・西田 睦
  • Organizer: 日本魚類学会
  • Place of Presentation: 神奈川県立 生命の星・地球博物館
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] 若狭湾で採集されたサケガシラ属魚類 (2014)
  • Author(s): 田城 文人・佐藤 崇・鈴木 啓太・佐藤 圭一
  • Organizer: 日本魚類学会
  • Place of Presentation: 神奈川県立 生命の星・地球博物館
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] ミトコンドリアゲノム全長配列から推定されたダルマガレイ科魚類の系統関係 (2012)
  • Author(s): 佐藤 崇，他2名
  • Organizer: 日本魚類学会
  • Place of Presentation: 山口県下関市水産大学校
• [Presentation] 国立科学博物館に所蔵する四国海盆や小笠原諸島近海で採集された深海魚標本 (2012)
  • Author(s): 篠原現人，中江雅典，佐藤 崇，他3名
  • Organizer: 日本魚類学会
  • Place of Presentation: 山口県下関市水産大学校
• [Presentation] Phylogeny of the lefteye flounders (family Bothidae): a mitogenomic perspective.
  • Author(s): Takashi P. Satoh, Masaki Miya and Mutsumi Nishida
  • Organizer: The 9th Indo-Pacific Fish Conference
  • Place of Presentation: Okinawa Convention Center
• [Presentation] 自然史標本からわかること ～標本を利用した研究例の紹介～
  • Author(s): 佐藤 崇
  • Organizer: 日本進化学会第１５回大会
  • Place of Presentation: 筑波大学
  • Invited