Structural basis of the filament formation of ESCRT-III proteins
Project/Area Number |
24770094
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | University of Toyama |
Principal Investigator |
OBITA TAKAYUKI 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 構造生物学 / 細胞分裂 / ESCRT / 古細菌 / タンパク質 |
Research Abstract |
We have cloned several ESCRT-III genes from Yeast or Archaea and made plasmids for protein expression. Although we have purified wild-type and mutants and tested crystallization, we have not got the good diffracting crystals, yet. We have determined the crystal structures of Archaeal CdvA, which interact with ESCRT-III proteins, and revealed that CdvA forms the filament structure. We have also shown the filament structure of CdvA using electron microscopy. Taken together, we have succeeded to show the structural basis of the filament structure of CdvA.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article]2012
Author(s)
Hadders MA, Agromayor M, Obita T, Perisic O, Caballe A, Kloc M, Lamers MH, Williams RL, Martin-Serrano J
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109(43)
Pages: 17424-9
Related Report
Peer Reviewed
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[Book] 生化学2014
Author(s)
帯田孝之
Total Pages
4
Publisher
公益社団法人日本生化学会
Related Report
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