X-ray crystallography of flavonoid metabolon

• Project/Area Number: 24770096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: MIZOHATA Eiichi 大阪大学, 工学(系)研究科(研究院), 助教 (90571183)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: X線結晶構造解析 / 構造生物学 / 国際情報交換

Outline of Final Research Achievements

Grapevine glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase, catalyzing the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. In this study, I determined crystal structures of VvGT5 in three kinds of structural states, and revealed structural basis of the sugar donor selectivity of the enzyme during catalysis.

Research Products

• [Journal Article] Structure-based design of a streptavidin mutant specific for an artificial biotin analogue (2015)
  • Author(s): Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A
  • Journal Title: The Journal of Biochemistry Volume: 157 Pages: 467-475
  • NAID: 40020489250
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Crystal structure of streptavidin mutant with low immunogenicity. (2015)
  • Author(s): Kawato T, Mizohata E, Meshizuka T, Doi H, Kawamura T, Matsumura H, Yumura K, Tsumoto K, Kodama T, Inoue T & Sugiyama A.
  • Journal Title: J Biosci Bioeng. Volume: 119(6) Issue: 6 Pages: 642-647
  • DOI: 10.1016/j.jbiosc.2014.10.025
  • NAID: 110009970751
  • Peer Reviewed / Open Access
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysisof UDP-glucuronic acid :flavonol-3-O-glucuronosyltransferase (2013)
  • Author(s): Mizohata, E., Okuda, T., Hatanaka, S., Nakayama, T., Horikawa, M., Nakayama, T., Ono, E., and Inoue, T.
  • Journal Title: (VvGT5) from the grapevine Vitis vinifera. Acta Cryst Volume: F69 Issue: 1 Pages: 65-68
  • DOI: 10.1107/s1744309112045095
  • Peer Reviewed
• [Presentation] ブドウの有用二次代謝物を合成する糖転移酵素の構造解析 (2013)
  • Author(s): 溝端栄一, 奥田卓馬, 畑中聖加, 中山泰亮, 松村浩由, 井上豪
  • Organizer: 第2回JACI/GSCシンポジウム（第13回 GSCシンポジウム）
  • Place of Presentation: メルパルク大阪
• [Presentation] Crystal structure of UDP-glucuronic acid:flavonol-3-Oglucuronosyltransferase from the grapevine Vitis vinifera (2012)
  • Author(s): Eiichi Mizohata, Takuma Okuda, Seika Hatanaka, Taisuke Nakayama, Manabu Horikawa, Toru Nakayama, Eiichiro Ono, Tsuyoshi Inoue
  • Organizer: AsCA 12/CRYSTAL 28
  • Place of Presentation: Adelaide, Australia