X-ray crystallography of Chlorella virus the molecular weight is over billion

• Project/Area Number: 24770097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: HIGASHIURA Akifumi 大阪大学, たんぱく質研究所, 助教 (90598129)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 構造生物学 / X線結晶構造解析 / Ｘ線結晶解析

Outline of Final Research Achievements

Chlorella virus is a large icosahedral virus of molecular weight over billion with the diameter of about 2000Å. It is reported that the virus has typical structures on the surface, the bi-layer lipid on the inside of the capsid and the genome encodes over 400 proteins. This study aimed to determine the atomic structure of Chlorella virus using by the techniques of X-ray crystallography. The high purity Chlorella virus was prepared on a large scale for the crystal structure analysis. The screening of the crystallization condition was carried out and small crystals were obtained. The fiber structures on the outer layer was clearly observed using the phase contrast cryo-electron microscopy.

Research Products

• [Journal Article] High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method (2013)
  • Author(s): Akifumi Higashiura, Kazunori Ohta, Mika Masaki, Masaru Sato, Koji Inaka, Hiroaki Tanaka and Atsushi Nakagawa
  • Journal Title: Journal of Synchrotron Radiation Volume: 20 Issue: 6 Pages: 989-993
  • DOI: 10.1107/s090904951302373x
  • Peer Reviewed
• [Presentation] SPring-8 BL44XU, Beamline Designed for a Structure Analysis of Large Biological Macromolecular Assemblies (2015)
  • Author(s): Akifumi Higashiura, Eiki Yamashita, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Takashi Kumasaka, Go Ueno, Masaki Yamamoto, Tomitake Tsukihara, Atsushi Nakagawa
  • Organizer: The 12th International Conference on Synchrotron Radiation Instrumentation
  • Place of Presentation: Marriott Marquis in Times Square, New York City
  • Year and Date: 2015-07-06 – 2015-07-10
• [Presentation] SPring-8生体超分子複合体構造解析ビームライン（大阪大学蛋白質研究所）BL44XUの現状 (2015)
  • Author(s): 山下栄樹、東浦彰史、吉村政人、長谷川和也、熊坂崇、古川行人、大端通、上野剛、山本雅貴、吉川信也、月原冨武、中川敦史
  • Organizer: 第２８回日本放射光学会年かい放射光科学合同シンポジウム
  • Place of Presentation: 立命館大学びわこ くさつキャンパス
  • Year and Date: 2015-01-10 – 2015-01-12
• [Presentation] 位相差クライオ電子顕微鏡によるクロレラウイルスの観察 (2014)
  • Author(s): 東浦彰史、宮崎直幸、村田和義、山本旭麻、原由美子、村上満理奈、中川敦史、山田隆
  • Organizer: 生理研研究会「電子顕微鏡機能イメージングの医学・生物学への応用」テーマ：「次世代の生物電顕を考える」
  • Place of Presentation: 生理学研究所
  • Year and Date: 2014-11-12 – 2014-11-13