X-ray crystallography of Chlorella virus the molecular weight is over billion
Project/Area Number |
24770097
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 構造生物学 / X線結晶構造解析 / X線結晶解析 |
Outline of Final Research Achievements |
Chlorella virus is a large icosahedral virus of molecular weight over billion with the diameter of about 2000Å. It is reported that the virus has typical structures on the surface, the bi-layer lipid on the inside of the capsid and the genome encodes over 400 proteins. This study aimed to determine the atomic structure of Chlorella virus using by the techniques of X-ray crystallography. The high purity Chlorella virus was prepared on a large scale for the crystal structure analysis. The screening of the crystallization condition was carried out and small crystals were obtained. The fiber structures on the outer layer was clearly observed using the phase contrast cryo-electron microscopy.
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Report
(4 results)
Research Products
(4 results)
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[Presentation] SPring-8 BL44XU, Beamline Designed for a Structure Analysis of Large Biological Macromolecular Assemblies2015
Author(s)
Akifumi Higashiura, Eiki Yamashita, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Takashi Kumasaka, Go Ueno, Masaki Yamamoto, Tomitake Tsukihara, Atsushi Nakagawa
Organizer
The 12th International Conference on Synchrotron Radiation Instrumentation
Place of Presentation
Marriott Marquis in Times Square, New York City
Year and Date
2015-07-06 – 2015-07-10
Related Report
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