Structural basis of mitochondrial proteins involved in anti-viral innate immunity

• Project/Area Number: 24770099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: KOSHIBA TAKUMI 九州大学, 理学(系)研究科(研究院), 准教授 (70403970)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ミトコンドリア / 抗ウイルス免疫 / MAVS / 分子間相互作用解析 / BRET / シグナル伝達 / 分子間相互作用

Research Abstract

Mitochondria-mediated antiviral innate immunity in mammals depends on the activation of RIG-I signaling pathway, and a mitochondrial membrane protein, MAVS plays an important role in the pathway. In the presence study, we found that the activated MAVS conformation is a highly ordered oligomer state by using BRET experiments. Our results reveal structural features underlying the regulation mechanism of MAVS signaling on the mitochondrial membrane.

Research Products

• [Journal Article] Mitochondrial protein mitofusin 2 is required for NLRP3 inflammasome activation after RNA virus infection. (2013)
  • Author(s): Ichinohe T, Yamazaki T, Koshiba T, Yanagi Y.
  • Journal Title: Proceeding National Academic Science USA Volume: 110(44) Issue: 44 Pages: 17963-17968
  • DOI: 10.1073/pnas.1312571110
  • Peer Reviewed
• [Journal Article] Transglutaminase-catalyzed protein-protein cross-linking suppresses the activity of the NF-κB-like transcription factor Relish. (2013)
  • Author(s): Shibata, T., Sekihara, S., Fujikawa, T., Miyaji, R., Maki, K., Ishihara, T., Koshiba, T., and Kawabata, S.
  • Journal Title: Science Signaling Volume: 6 Issue: 285 Pages: 1-9
  • DOI: 10.1126/scisignal.2003970
  • Peer Reviewed
• [Journal Article] Fis1 acts as mitochondrial recruitment of TBC1D15 that involved in regulation of mitochondrial morphology (2013)
  • Author(s): K. Onoue et al.
  • Journal Title: J. Cell Sci. Volume: 126 Issue: 1 Pages: 176-186
  • DOI: 10.1242/jcs.111211
  • Peer Reviewed
• [Journal Article] A structural perspective of the MAVS-regulatory mechanism on the mitochondrial outer membrane (2013)
  • Author(s): Sasaki, O., Yoshizumi, T., Kuboyama, M., Ishihara, T., Suzuki, E., Kawabata, S., and *Koshiba, T.
  • Journal Title: Biochim. Biophys. Acta - Mol. Cell Res. Volume: 1833 Issue: 5 Pages: 1017-1027
  • DOI: 10.1016/j.bbamcr.2013.01.010
  • Peer Reviewed
• [Journal Article] Mitochondrial-mediated antiviral immunity (2013)
  • Author(s): *Koshiba, T.
  • Journal Title: Biochim. Biophys. Acta - Mol. Cell Res. Volume: 1833 Issue: 1 Pages: 225-232
  • DOI: 10.1016/j.bbamcr.2012.03.005
  • Peer Reviewed
• [Journal Article] ミトコンドリアと抗ウイルス免疫 (2013)
  • Author(s): 小柴琢己
  • Journal Title: 生化学 Volume: 85 Pages: 336-344
  • NAID: 10031177325
• [Journal Article] Fis1 acts as mitochondrial recruitment factor for TBC1D15 that involved in regulation of mitochondrial morphology (2013)
  • Author(s): Onoue, K., Jofuku, A., Ban-Ishihara, R., Ishihara, T., Maeda, M., Koshiba, T., Itoh, T., Fukuda, M., Otera, H., Oka, T., Takano, H., Mizushima, N., Mihara, K., and Ishihara, N.
  • Journal Title: J. Cell Sci. Volume: 126 Pages: 176-185
  • Peer Reviewed
• [Journal Article] Microbe-specific c3b deposition in the horseshoe crab complement system in a c2/factor B-dependent or-independent manner (2012)
  • Author(s): Tagawa K, Yoshihara T, Shibata T, Kitazaki K, Endo Y, Fujita T, Koshiba T and Kawabata S.
  • Journal Title: PLoS One Volume: 7 Issue: 5 Pages: e36783-e36783
  • DOI: 10.1371/journal.pone.0036783
  • Peer Reviewed
• [Presentation] Mitochondria and antiviral signaling (2013)
  • Author(s): Koshiba, T
  • Organizer: The 4th International Symposium on Dynamics of Mitochondria (DynaMito2013) (Organizers ; Prof. Koji Okamoto and Prof. Michael Ryan)
  • Place of Presentation: Okinawa, Japan
• [Presentation] ミトコンドリアを介した抗ウイルスシグナル伝達 (2013)
  • Author(s): 小柴琢己
  • Organizer: 第86回日本生化学会大会(会頭 ; 宮園浩平教授)
  • Place of Presentation: パシフィコ横浜, 横浜
• [Presentation] Mitochondria and antiviral signaling (2013)
  • Author(s): Koshiba, T.
  • Organizer: The 4th International Symposium on Dynamics of Mitochondria (DynaMito2013)
  • Place of Presentation: Okinawa, JAPAN
  • Invited
• [Presentation] ミトコンドリアを介した抗ウイルスシグナル伝達 (2013)
  • Author(s): 小柴琢己
  • Organizer: 第86回 日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] ミトコンドリア・ダイナミクスの作用機序とその生理的意義 (2012)
  • Author(s): 小柴琢己
  • Organizer: 日本生化学会平成24年度奨励賞受賞講演(会頭 ; 藤木幸夫教授)
  • Place of Presentation: 福岡国際会議場, 福岡
• [Presentation] Mitochondrial-mediated antiviral immunity (2012)
  • Author(s): 小柴琢己
  • Organizer: 第85回日本生化学会大会(会頭 ; 藤木幸夫教授)
  • Place of Presentation: 福岡国際会議場, 福岡
• [Presentation] MAVS signaling and its inhibition (2012)
  • Author(s): Koshiba, T
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012 (IEIIS2012) (President; Prof. Kensuke Miyake)
  • Place of Presentation: Tokyo, Japan
• [Presentation] Antiviral innate immunity and mitochondrial function (2012)
  • Author(s): Koshiba, T
  • Organizer: The 2nd UCL-JSPS international Symposium"Mitochondria - from the Fundamental Aspects to Medical Importance"(Organizer; Dr. Takehiro Yasukawa)
  • Place of Presentation: UCL Wilkins Building, London, UK
• [Presentation] ミトコンドリアの膜ダイナミクス制御とその生理機能的役割に関する研究 (2012)
  • Author(s): 小柴琢己
  • Organizer: 第14回花王研究奨励賞受賞記念講演(主催;公益財団法人花王芸術・科学財団)
  • Place of Presentation: 花王すみだ事業所, 東京
• [Presentation] ミトコンドリアの自然免疫における役割解明 (2012)
  • Author(s): 小柴琢己
  • Organizer: 平成24年度日本生化学会九州支部例会学術奨励賞・受賞講演(支部長 ; 寺田成之教授)
  • Place of Presentation: 福岡大学, 福岡
• [Presentation] MAVS signaling and its inhibition (2012)
  • Author(s): Takumi Koshiba
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012 (IEIIS2012)
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] Antiviral innate immunity and mitochondrial function (2012)
  • Author(s): Takumi Koshiba
  • Organizer: The 2nd UCL-JSPS international Symposium “Mitochondria - from the Fundamental Aspects to Medical Importance
  • Place of Presentation: London 英国
  • Invited
• [Presentation] Mitochondrial-mediated antiviral immunity (2012)
  • Author(s): 小柴琢己
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ミトコンドリア・ダイナミクスの作用機序とその生理的意義 (2012)
  • Author(s): 小柴琢己
  • Organizer: 日本生化学会 平成24年度奨励賞受賞講演
  • Place of Presentation: 福岡
  • Invited
• [Presentation] ミトコンドリアの自然免疫における役割解明 (2012)
  • Author(s): 小柴琢己
  • Organizer: 平成24年度 日本生化学会九州支部例会 学術奨励賞・受賞講演
  • Place of Presentation: 福岡
  • Invited
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~koshiba/index.html