Structural biology study of the ER-associated degradation machinery based on eukaryotic thermophile genomic information
Project/Area Number |
24770102
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
|
Research Institution | Nagoya City University |
Principal Investigator |
SATOH Tadashi 名古屋市立大学, 薬学研究科(研究院), 准教授 (80532100)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 好熱性真核生物 / 立体構造解析 / PDI / グルコシダーゼ / 構造生物学 / X線結晶構造解析 / NMR解析 |
Research Abstract |
In this study, we aimed to understand the molecular recognition and operating mechanisms of quality control machineries responsible for the glycoprotein fate determination (folding, transport, and degradation) in the endoplasmic reticulum. Our distinctive approach includes application of the genome information of eukaryotic thermophiles that can grow at high temperature (about 60 degrees Celsius). Using X-ray crystallography, we successfully determined the first crystal structures of protein disulfide isomerase (PDI) catalyzing disulfide bond formation and isomerization, and the catalytic domain of glycoprotein processing enzyme glucosidase II.
|
Report
(3 results)
Research Products
(47 results)
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[Journal Article] Structural Basis for Proteasome Formation Controlled by an Assembly Chaperone Nas22014
Author(s)
Satoh T, Saeki Y, Hiromoto T, Wang YH, Uekusa Y, Yagi H, Yoshihara H, Yagi-Utsumi M, Mizushima T, Tanaka K, Kato K
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Journal Title
Structure
Volume: (掲載確定)
Issue: 5
Pages: 731-743
DOI
Related Report
Peer Reviewed
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[Journal Article] ATPase activity and ATP-dependent conformational change in the co-chaperone HSP70/HSP90-organizing protein (HOP)2014
Author(s)
S. Yamamoto, GP. Subedi, S. Hanashima, T. Satoh, M. Otaka, H. Wakui, K. Sawada, S. Yokota, Y. Yamaguchi, H. Kubota, H. Itoh
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Journal Title
J. Biol. Chem.
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] ATPase activity and ATP-dependent conformational change in the co-chaperone HOP.2014
Author(s)
Yamamoto S, Subedi GP, Hanashima S, Satoh T, Otaka M, Wakui H, Sawada KI, Yokota SI, Yamaguchi Y, Kubota H, Itoh H.
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Journal Title
Journal of Biological Chemistry
Volume: 289
Issue: 14
Pages: 9880-9886
DOI
Related Report
Peer Reviewed
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