Dynamic behavior of human VRK1 kinase in living cells
| Project/Area Number |
24770108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tohoku University (2014)
Institute of Physical and Chemical Research (2012-2013) |
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Principal Investigator |
KOSHIBA Seizo 東北大学, 東北メディカル・メガバンク機構, 准教授 (70332301)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 酵素 / キナーゼ / NMR / In-cell / In-Cell NMR / 19F-NMR / 動的構造 / VRK1 |
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| Outline of Final Research Achievements |
I developed a new method to analyze dynamic behavior of proteins in living cells using In-Cell NMR and fluorine-labeling methods. High-resolution 19F-NMR spectra of VRK1 kinase protein were obtained. This method is useful for understanding the dynamic regulation of proteins under physiological conditions.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Solutionstructure and siRNA-mediated knockdown analysis of the mitochondorial disease-related protein C12orf652012
Author(s)
Kogure H, Hikawa Y, Hagihara M, Tochio N, Koshiba S, Inoue Y, Guntert P, Kigawa, T, Yokoyama S, and Nameki N
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Journal Title
Proteins
Volume: 80
Issue: 11
Pages: 2629-2642
DOI
Related Report
Peer Reviewed
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