Dynamic behavior of human VRK1 kinase in living cells

Research Project

Project/Area Number	24770108
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Tohoku University (2014) Institute of Physical and Chemical Research (2012-2013)
Principal Investigator	KOSHIBA Seizo 東北大学, 東北メディカル・メガバンク機構, 准教授 (70332301)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	酵素 / キナーゼ / NMR / In-cell / In-Cell NMR / 19F-NMR / 動的構造 / VRK1
Outline of Final Research Achievements	I developed a new method to analyze dynamic behavior of proteins in living cells using In-Cell NMR and fluorine-labeling methods. High-resolution 19F-NMR spectra of VRK1 kinase protein were obtained. This method is useful for understanding the dynamic regulation of proteins under physiological conditions.