Structural study of heme transporter from pathogenic bacteria

Research Project

Project/Area Number	24770110
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	NAOE Youichi 独立行政法人理化学研究所, 加藤分子物性研究室, 特別研究員 (70464146)
Project Period (FY)	2012 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	ヘム / 膜タンパク質 / ABCトランスポーター / 結晶 / ABC トランスポーター / X 線結晶構造解析
Research Abstract	Pathogenic bacteria sequester heme from the infected host as an iron source. Heme transporter is the membrane protein used to transport heme into the cytoplasm. To further understanding of the transport mechanism, I have pursued X-ray crystallographic analysis. Through trial and error, 3.5 Å resolution crystal of heme transporter was obtained. In addition, colored crystal thought to be heme-bound has been obtained.

Report

(3 results)