| Project/Area Number |
24770111
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
NAGAE Masamichi 独立行政法人理化学研究所, 糖鎖構造生物学研究チーム, 研究員 (60619873)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | X線結晶構造解析 / 生物物理 / 糖鎖 / 蛋白質 / 糖鎖生物学 |
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| Research Abstract |
The immune system is a system which discriminates and removes the pathogens and highly developed in mammals. Groups of pathogens share similar chemical structures known as pathogen-associated molecular pathogens (PAMPs). Many cell-surface receptors which involve in innate immunity specifically recognize PAMPs. Especially, C-type lectin receptors (CTLR) bind to carbohydrates derived from pathogens such as bacteria, virus and fungi. Both DCIR-2 and BDCA-2 belong to CTLRs and are highly expressed on dendritic cells. DCIR-2 works as negative regulator, while BDCA-2 works as activator. Our purpose is to clarify the ligand recognition of both proteins. We initially determined the crystal structures of DCIR-2 in the absence and complexed with its specific ligand, bisected N-glycan. As a result, non-conserved amino acids enable DCIR-2 to bind to bisected glycan. Next we solved the crystal structure of BDCA-2 in unliganded form. BDCA-2 forms 'domain-swapped' dimer in various conditions.
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