| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The aim of this study is clarifying the molecular machinery of lysophospholipid signaling using zebrafish. Aberrant lysophosphatidic acid signaling is involved in multiple diseases including cancer and pulmonary fibrosis, although the how the excessive lysophosphatidic acid signaling cause these diseases is still unknown. We overexpressed the lysophosphatidic acid-producing enzyme, autotaxin, in zebrafish embryo. Unexpectedly, the embryo displayed cardia bifida phenotype, that is rescued by down-regulating the lysophosphatidic acid signaling by lysophosphatidic acid receptor morpholino. Cardia bifida phenotype is typically observed in the hypo-sphingosine 1-phosphate signaling embryo, and therefore, excessive lysophosphatidic acid signaling may be involved in down regulating the sphingosine 1-phosphate signaling.
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