analysis of spacio-temporal lysophospholipid signaling in zebrafish embryo
| Project/Area Number |
24770114
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
HAMA Kotaro 帝京大学, 薬学部, 講師 (20534481)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 脂質 |
|---|
| Outline of Final Research Achievements |
The aim of this study is clarifying the molecular machinery of lysophospholipid signaling using zebrafish. Aberrant lysophosphatidic acid signaling is involved in multiple diseases including cancer and pulmonary fibrosis, although the how the excessive lysophosphatidic acid signaling cause these diseases is still unknown. We overexpressed the lysophosphatidic acid-producing enzyme, autotaxin, in zebrafish embryo. Unexpectedly, the embryo displayed cardia bifida phenotype, that is rescued by down-regulating the lysophosphatidic acid signaling by lysophosphatidic acid receptor morpholino. Cardia bifida phenotype is typically observed in the hypo-sphingosine 1-phosphate signaling embryo, and therefore, excessive lysophosphatidic acid signaling may be involved in down regulating the sphingosine 1-phosphate signaling.
|
Report
(4 results)
Research Products
(15 results)
-
[Journal Article] Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo2014
Author(s)
Nakanaga K, Hama K, Kano K, Sato T, Yukiura H, Inoue A, Saigusa D, Tokuyama H, Tomioka Y, Nishina H, Kawahara A, Aoki J
-
Journal Title
J. Biochem
Volume: 155
Issue: 4
Pages: 235-241
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Molecular species of phospholipids with very long chain fatty acids in skin fibroblasts of Zellweger syndrome.2013
Author(s)
Hama K, Nagai T, Nishizawa C, Ikeda K, Morita M, Satoh N, Nakanishi H, Imanaka T, Shimozawa N, Taguchi R, Inoue K, Yokoyama K.
-
Journal Title
Lipids
Volume: 48
Issue: 12
Pages: 1253-1267
DOI
Related Report
Peer Reviewed
-
[Journal Article] Simultaneous quantitation of sphingoid bases and their phosphates in biological samples by liquid chromatography/electrospray ionization tandem mass spectrometry2012
Author(s)
Saigusa D, Shiba K, Inoue A, Hama K, Okutani M, Iida N, Saito M, Suzuki K, Kaneko T, Suzuki N, Yamaguchi H, Mano N, Goto J, Hishinuma T, Aoki J, Tomioka Y
-
Journal Title
Anal Bioanal Chem
Volume: 403巻
Issue: 7
Pages: 1897-1905
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
