Physiological function of PIK3C3

• Project/Area Number: 24770115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Akita University
• Principal Investigator: EGUCHI SATOSHI 秋田大学, 医学(系)研究科(研究院), 助教 (70457117)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イノシトールリン脂質

Research Abstract

To investigate the physiological function of PIK3C3, we generated T cell specific deletion of PIK3C3 mice. These mice caused autoimmune disease and inflammation in several organs, and died for severe bronchopneumonia. We showed increase of IL-17A production by promotion of Th17 differentiation in PIK3C3 deficient naive T cell was the cause of disease. These results suggested that PIK3C3 suppressed Th17 differentiation and autoimmune disease.

Research Products

• [Journal Article] A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A (2013)
  • Author(s): Nakashima, A., Tanimura-Ito, K., Oshiro, N., Eguchi, S., Miyamoto, K., Momonami, A., Kamada, S., Kikkawa, U. and Yonezawa, K.
  • Journal Title: FEBS letter Volume: 587 Issue: 18 Pages: 2924-2929
  • DOI: 10.1016/j.febslet.2013.07.027
  • Peer Reviewed
• [Journal Article] Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes (2013)
  • Author(s): Nakashima A, Kawanishi I, Eguchi S, Yu EH, Eguchi S, Oshiro N, Yoshino K, Kikkawa U, Yonezawa K.
  • Journal Title: Journal of Biomedical Science Volume: 20 Issue: 1 Pages: 24-24
  • DOI: 10.1186/1423-0127-20-24
  • NAID: 120005460275
  • Peer Reviewed
• [Journal Article] Deletion of autophagy-related 5 (Atg5) and Pik3c3 genes in the lens causes cataract independent of programmed organelle degradation. (2013)
  • Author(s): 森下英晃、江口賢史、木村洋貴、佐々木純子、酒巻有里子、Robinson ML、佐々木雄彦、水島昇
  • Journal Title: J Biol Chem. Volume: 288 Issue: 16 Pages: 11436-47
  • DOI: 10.1074/jbc.m112.437103
  • Peer Reviewed
• [Journal Article] SHIP1 deletion enhances ROS production and decrease survival of the S. aureus infected mice (2013)
  • Author(s): Chida, S., Ito, R., Kofuji, S., Eguchi, S., Takasuga, S., Sasaki, J. and Sasaki, T.
  • Journal Title: Akita Journal of medicine Volume: 39 Pages: 95-101
  • NAID: 110009575396
  • Peer Reviewed
• [Journal Article] SHIP1 deletion enhances ROS production and decreases survival of the S. aureus infected mice (2013)
  • Author(s): Chida S, Itoh R, Kofuji S, Eguchi S, Takasuga S, Sasaki J, Sasaki T
  • Journal Title: Akita Journal of medicine Volume: 39 Pages: 95-101
  • NAID: 110009575396
  • Peer Reviewed
• [Journal Article] Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine (2012)
  • Author(s): Takasuga , Wada et al.
  • Journal Title: PNAS 110 Volume: 1726-1731 Issue: 5 Pages: 1726-31
  • DOI: 10.1073/pnas.1213212110
  • Peer Reviewed
• [Presentation] 脂質キナーゼPIK3C3欠損による自己免疫疾患 (2013)
  • Author(s): 江口賢史、木村洋貴、浅沼研、堀江泰夫、佐々木純子、佐々木雄彦
  • Organizer: 第11回北東北血液研究会
  • Place of Presentation: 秋田市
  • Year and Date: 2013-04-27
• [Presentation] リン脂質キナーゼPIK3C3欠損による自己免疫疾患
  • Author(s): 江口賢史、木村洋貴、浅沼研、堀江泰夫、佐々木純子、佐々木雄彦
  • Organizer: 第１１回北東北血液研究会
  • Place of Presentation: 秋田県秋田市
• [Remarks] 秋田大学大学院医学系研究科病態医学制御学系微生物学講座
  • URL: http://www.med.akita-u.ac.jp/~bisei/index.html