| Project/Area Number |
24770117
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ARAIE Hiroya 筑波大学, 生命環境系, 特任助教 (30596169)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | セレン / トランスポーター / セレン含有化合物 / Emiliania huxleyi / リポソーム / 微量元素 / 輸送体 / 円石藻 / セレノシステイン |
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| Research Abstract |
There is only trace amounts of essential element selenium (Se) in environment. Therefore, microalgae thought to possess specific Se transporter for efficient utilization of Se. I tried to identify this Se transporter. To date, there are no reports of transporters specific for selenite. This time, I aimed at building activity measurement system for the identification. Considering the fact that the nucleotide sequence of the algae is GC rich and the transporter is a membrane protein, I tried expression analysis by using a cell-free system. As a result, it was possible to synthesize a membrane protein by using liposomes as basis. In addition, I have found that there may be a similarity between Se compounds in various microalgae. Therefore, it is expected to identify Se transporter that uses algae that can be genetically engineered for knockdown assay.
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