Mechanism of large protein secretion regulated by small GTPase and complex localized at the ER
| Project/Area Number |
24770119
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAITO Kota 東京大学, 薬学研究科(研究院), 助教 (60549632)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | コラーゲン / 分泌 / 小胞体 / COPII / 低分子量G蛋白質 / 低分子量Gタンパク質 |
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| Research Abstract |
Collagen synthesized in the ER is too big to fit into conventional transport COPII carriers. How collagen exports from the ER is still unclear. We have previously identified cTAGE5/TANGO1 complex as a cargo receptor for collagen VII at ER exit sites. Here we show that cTAGE5 interacts with Sec12, a guanine-nucleotide exchange factor of Sar1 at ER exit sites. Upon cTAGE5 depletion, Sec12 delocalizes from the ER exit sites. These results imply that collagen VII secretion from the ER is regulated not only by cTAGE5/TANGO1 complex but with the activity of Sar1 small GTPase.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] rl8/ARL-8 functions in apoptotic cell removal by mediating phagolysosome formation in C. elegans.2013
Author(s)
Sasaki A, Nakae I, Nagasawa M, Hashimoto K, Abe F, Saito K, Fukuyama M, Gengyo-Ando K, Mitani S, Katada T, Kontani K.
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Journal Title
Mol. Biol. Cell
Volume: 24
Issue: 10
Pages: 1584-1592
DOI
Related Report
Peer Reviewed / Open Access
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