Clarification of a novel function of recycling endosomes with localization of Ras and its activation mechanism

Research Project

Project/Area Number	24770123
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Functional biochemistry
Research Institution	Osaka University
Principal Investigator	MISAKI Ryo 大阪大学, 生物工学国際交流センター, 講師 (20571186)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	リサイクリングエンドソーム / Ras / シグナル伝達
Research Abstract	Recycling endosomes, REs, have been known to regulate not only intracellular recycling pathways but also the signal transduction. In this study, an amino acid sequence which contributes the steady-state distribution of Ras small GTPase to REs was determined. It is also demonstrated that a Ras-activating factor co-localizes with activated-Ras on REs by extracellular stimulation and a suppression of expression of Ras-activating factor causes depletion of Ras-activation on REs. These results suggest that RE is an organelle with function of not only intracellular traffic but also signal transduction. It is expected that more detailed studies of REs give new approaches for cancer therapy.