Molecular mechanism of crystallization enzyme

• Project/Area Number: 24770124
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: ISHIKAWA Haruto 大阪大学, 理学(系)研究科(研究院), 講師 (40551338)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 金属タンパク質 / ヘム / 酵素 / マラリア

Research Abstract

Heme detoxification protein (HDP), potent in converting heme into an insoluble crystalline form called hemozoin (Hz), was found in the malaria parasite. We succeeded in the identification of the critical amino acid residues for the Hz formation by using the site-directed mutagenesis, ESR, and resonance Raman measurements. We also investigated that the active site residues in alpha - glucosidase (AGLU) in a blood-sucking bug. Spectroscopic characterization of the recombinant AGLU revealed that a histidine residue exists in the active site.

Research Products

• [Journal Article] Ultraviolet resonance Raman observations of the structural dynamics of rhizobial oxygen sensor FixL on ligand recognition (2013)
  • Author(s): Shinji Yano, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, Yasuhisa Mizutani
  • Journal Title: Journal of Physical Chemistry B Volume: 117 Issue: 49 Pages: 15786-15791
  • DOI: 10.1021/jp406709e
  • Peer Reviewed
• [Journal Article] Intersubunit communication via changes in hemoglobin quaternary structures revealed by time-resolved resonance Raman spectroscopy: Direct observation of the Perutz mechanism (2013)
  • Author(s): Kenta Yamada, Haruto Ishikawa, Misao Mizuno, Naoya Shibayama, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 117 Issue: 41 Pages: 12461-12468
  • DOI: 10.1021/jp407735t
  • Peer Reviewed
• [Journal Article] Heme-binding properties of heme detoxification protein from Plasmodium falciparum (2013)
  • Author(s): Keisuke Nakatani, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani
  • Journal Title: Biochem Biophys Res Commun Volume: 439 Issue: 4 Pages: 477-480
  • DOI: 10.1016/j.bbrc.2013.08.100
  • Peer Reviewed
• [Journal Article] Structural Basis for Oxygen Sensing and Signal Transduction of the Heme-Based Sensor Protein Aer2 from Pseudomonas aeruginosa (2012)
  • Author(s): H. Sawai, H. Sugimoto, Y. Shiro, H. Ishikawa, Y. Mizutani, S. Aono
  • Journal Title: Chem. Commun Volume: 48 Issue: 52 Pages: 6523-6525
  • DOI: 10.1039/c2cc32549g
  • Peer Reviewed
• [Journal Article] Structural dynamics of proximal heme pocket in HemAT-Bs associated with O2 dissociation (2012)
  • Author(s): Y. Yoshida, H. Ishikawa, S. Aono, Y. Mizutani
  • Journal Title: Biochem. Biophys. Acta - Proteins and Proteomics Volume: 1824 Issue: 7 Pages: 866-872
  • DOI: 10.1016/j.bbapap.2012.04.007
  • Peer Reviewed
• [Journal Article] Structural dynamics of proximal heme pocket in HemAT-Bs associated with oxygen dissociation (2012)
  • Author(s): Yuu Yoshida, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani
  • Journal Title: Biochimica et Biophysica Acta Volume: 1824 Pages: 866-872
  • Peer Reviewed
• [Presentation] 酸素センサータンパク質FixLのリガンド特異的な構造変化 : 部位特異的変異と紫外共鳴ラマン分光法による研究 (2014)
  • Author(s): 山脇竹生、石川春人、水野操、中村寛夫、城宜嗣、水谷泰久
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
• [Presentation] Oxygen-dependent Allosteric Control of Heme Proteins (2014)
  • Author(s): 石川春人
  • Organizer: The 1st Workshop of Studying the Function of Soft Molecular Systems by the Concerted Use of Theory and Experiment, "What is soft molecular system?"
  • Place of Presentation: ホテル紅や、長野
  • Invited
• [Presentation] Protein dynamics of CooA upon CO dissociation studied by time-resolved resonance Raman spectroscopy (2013)
  • Author(s): 大友章裕、石川春人、水野操、青野重利、水谷泰久
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都
• [Presentation] Heme binding site in Rhodnius prolixusα-glucosidase promoting the heme crystallization (2013)
  • Author(s): 加来祥太郎、中谷圭佑、石川春人、水谷泰久
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都
• [Presentation] Structural changes in the heme and heme pocket upon CO dissociation of CooA observed by time-resolved resonance Raman spectroscopy (2013)
  • Author(s): Akihiro Otomo, Haruto Ishikawa, Misao Mizuno, Shigetoshi Aono, Yasuhisa Mizutani
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Identification of active-site residues in heme detoxification protein (2013)
  • Author(s): Haruto Ishikawa, Keisuke Nakatani, Shigetoshi Aono, Yasuhisa Mizutani
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Heme Detoxification Proteinによるヘム結晶化機構 (2013)
  • Author(s): 石川春人、中谷圭佑、青野重利、水谷泰久
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪
• [Presentation] ヘミンの結晶化触媒活性を有するサシガメ由来α-グルコシダーゼの分光学的研究 (2013)
  • Author(s): 加来祥太郎、中谷圭佑、石川春人、水谷泰久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 滋賀
• [Presentation] 紫外共鳴ラマン分光法による酸素センサータンパク質FixLのリガンド特異的な構造変化の観測 (2013)
  • Author(s): 山脇竹生、石川春人、水野操、中村寛夫、城宜嗣、水谷泰久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 滋賀
• [Presentation] 時間分解共鳴ラマン分光法を用いた酸素センサータンパク質HemATの酸素脱離に伴う構造変化観測 (2013)
  • Author(s): 石川春人、吉田祐、青野重利、水谷泰久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 滋賀
• [Presentation] Heme Detoxification Protein によるヘム結晶化機構 (2013)
  • Author(s): 石川春人、中谷圭佑、青野重利、水谷泰久
  • Organizer: 第40回生体分子科学討論会
  • Place of Presentation: 大阪大学、吹田
• [Presentation] Identification of Active-site Residues in Heme Detoxification Protein (2013)
  • Author(s): 石川春人、中谷圭佑、青野重利、水谷泰久
  • Organizer: 16th International Conference on BioInorganic Chemistry
  • Place of Presentation: Alpexpo center, Grenoble, France
• [Presentation] 時間分解共鳴ラマン分光法を用いた酸素センサータンパク質HemATの酸素脱離に伴う構造変化観測 (2013)
  • Author(s): 石川 春人、吉田 祐、青野 重利、水谷 泰久
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] Hemozoin formation mechanism of heme detoxification protein in malaria parasite (2012)
  • Author(s): Keisuke Nakatani, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada Conference Centre
• [Presentation] ヘモゾイン形成機構におけるHeme Detoxification Proteinとヘムの相互作用の解明 (2012)
  • Author(s): 中谷 圭佑、石川 春人、青野 重利、水谷 泰久
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks] 大阪大学水谷研究室ホームページ
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks] 大阪大学水谷研究室ホームページ
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html