Elucidation of mechanism of molecular dynamics recognition by RNA machineries involved in the thermostable RNA maturation
| Project/Area Number |
24770125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Ehime University |
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Principal Investigator |
HIRATA Akira 愛媛大学, 理工学研究科, 講師 (60527381)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 酵素の基質認識機構 / 酵素の生理機能 / tRNAプロセシング / アーキア / RNA成熟マシナリー / アーキア（古細菌） / 構造生物学 / 遺伝学 / 生化学 / RNA成熟 |
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| Research Abstract |
Structural and functional analyses of tRNA splicing enzyme (EndA) from ultra-small archaeon, ARMAN-2, revealed that ARMAN-2 specific loop is involved in the broad substrate specificity of ARMAN-2 EndA. On the other hands, structural, biochemical and genetic analyses of tRNA (m2G10/m22G10) methyltransferase (arcTrm11) in the hyperthermophilic archaeon Thermococcus kodakarensis showed that the crystal structure of arcTrm11 consists of two domains, N-terminal THUMP domain which is involved in the RNA recognition and C-terminal Rossman fold-type catalytic domain, and that arcTrm11 gene is essential for cell viability at high temperature.
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Report
(3 results)
Research Products
(12 results)
