Quality control mechanisms for aberrant mRNA that emerges under stress conditions

• Project/Area Number: 24770131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Nagoya City University
• Principal Investigator: HOSODA Nao 名古屋市立大学, 薬学研究科(研究院), 講師 (40438198)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: mRNA分解 / 翻訳 / ストレス / ストレス応答 / 核酸

Research Abstract

We further confirmed quality control mechanisms for non-stop mRNA using mammalian cells. The non-stop mRNA is degraded in a translation-dependent manner. The decay requires exosome complex. In addition, we examined decay of in vitro synthesized mRNA, which is an example of aberrant mRNA. The decay does not depend on poly(A) tail shortening. The exosome functions in the mRNA decay as in the non-stop mRNA decay.

Research Products

• [Journal Article] Anti-proliferative protein Tob directly regulates c-myc proto-oncogene expression through cytoplasmic polyadenylation element-binding protein CPEB (2014)
  • Author(s): Koichi Ogami, Nao Hosoda, Yuji Funakoshi, and Shin-ichi Hoshino
  • Journal Title: Oncogene Volume: 33 Pages: 55-64
• [Journal Article] The processed isoform of the translation termination factor eRF3 localizes to the nucleus to interact with the ARF tumor suppressor (2014)
  • Author(s): Yoshifumi Hashimoto, Naomichi Kumagai, Nao Hosoda, and Shin-ichi Hoshino
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 445 Issue: 3 Pages: 639-644
  • DOI: 10.1016/j.bbrc.2014.02.063
  • Peer Reviewed
• [Journal Article] The Hbs1-Dom34 protein complex functions in non-stop mRNA decay (NSD) in mammalian cells (2013)
  • Author(s): Shyuhei Saito, Nao Hosoda, and Shin-ichi Hoshino
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 17832-17843
• [Journal Article] The Hbs1-Dom34 protein complex functions in non-stop mRNA decay (NSD) in mammalian cells. (2013)
  • Author(s): Shyuhei Saito, Nao Hosoda, and Shin-ichi Hoshino
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 24 Pages: 17832-17843
  • DOI: 10.1074/jbc.m112.448977
  • Peer Reviewed
• [Journal Article] Anti prolifetative protein Tob directly regulates c-myc proto-oncogene expression through cytoplasmic polyadenylation element-binding protein CPEB. (2013)
  • Author(s): Ogami, K., Hosoda, N., Funakoshi, N., Hoshino, S.
  • Journal Title: Oncogene Volume: - Issue: 1 Pages: 55-64
  • DOI: 10.1038/onc.2012.548
  • Peer Reviewed
• [Journal Article] Translation termination factor eRF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apotosis (2012)
  • Author(s): Yoshifumi Hashimoto, Nao Hosoda, Pinaki Datta, Emad S. Alnemri, and Shin-ichi Hoshino
  • Journal Title: Apotosis Volume: 17 Pages: 1287-1299
• [Journal Article] Biological Role of the Two Overlapping Poly(A)-binding Protein Interacting Motifs 2 (PAM2) of a Eukaryotic Releasing Factor, eRF3 in mRNA decay (2012)
  • Author(s): Masanori Osawa, Nao Hosoda, Tamiji Nakanishi, Naoyuki Uchida, Tomomi Kimura, Shunsuke Imai, Asako Machiyama, Toshiaki Katada, Shin-ichi Hoshino, and Ichio Shimada
  • Journal Title: RNA Volume: 18 Pages: 1957-1967
• [Journal Article] Translation termination factor eRF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apoptosis. (2012)
  • Author(s): Hashimoto, Y., Hosoda, N, Datta, P., Alnemri, ES., Hoshino, S.
  • Journal Title: Apoptosis Volume: 17 Issue: 12 Pages: 1287-1299
  • DOI: 10.1007/s10495-012-0765-7
  • Peer Reviewed
• [Presentation] CPEBによって制御されるmRNAポリA鎖分解機構Mechanisms of a transcript-specific mRNA deadenylation by CPEB (2013)
  • Author(s): 細田直、釣真由美、尾上耕一、星野真一
  • Organizer: 第36回日本分子生物学会年会(ワークショップ)
  • Place of Presentation: 神戸
• [Presentation] 翻訳終結因子eRF3のプロセス体p-eRF3の新規機能 (2013)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] B型肝炎治療を目指したmRNAとランスフェクションによる高効率遺伝子発現系の確立 (2013)
  • Author(s): 野木森拓人、細田直、星野真一
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2013
  • Place of Presentation: 鈴鹿
• [Presentation] 哺乳動物細胞におけるノンストップmRNA分解の分子機構の解析 (2013)
  • Author(s): 細田直、齊藤修平、星野真一
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 翻訳終結因子eRF3の細胞内局在制御 (2013)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 翻訳終結因子Sup35の切断が酵母プリオンの出現を防御する (2013)
  • Author(s): 岡本淳志、細田直、星野真一
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] eRF3ファミリーに属するGタンパク質GTPBP1の機能解析 (2013)
  • Author(s): 西浦久達、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] ポリA鎖分解酵素NocturninによるmRNA動態制御 (2013)
  • Author(s): 奥村真由、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] 切断型eRF3によるアポトーシス阻害タンパク質IAPを介したアポトーシス制御機構の解析 (2013)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] Mechanisms of a transcript-specific mRNA deadenylation by CPEB (2013)
  • Author(s): 細田 直、釣 真由美、尾上 耕一、星野 真一
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 哺乳動物細胞におけるノンストップ mRNA 分解の分子機構の解析 (2013)
  • Author(s): 細田 直、齊藤 修平、星野 真一
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 翻訳終結因子Sup35の切断が酵母プリオンの出現を防御する (2013)
  • Author(s): 岡本 淳志、細田 直 、星野 真一
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 翻訳終結因子eRF3の細胞内局在制御 (2013)
  • Author(s): 橋本 芳史、細田 直、星野 真一
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] テロメラーゼRNA(TLC1)の成熟化機構の解析 (2012)
  • Author(s): 田中麻記子、細田直、星野真一
  • Organizer: 第85回生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Anti-proliferative protein Tob directly regulates c-myc proto-oncogene expression through cytoplasmic polyadenylation element-binding protein CPEB (2012)
  • Author(s): Koichi Ogami, Nao Hosoda, Yuji Funakoshi, and Shin-ichi Hoshino
  • Organizer: The Complex Life of mRNA, EMBO Symposia
  • Place of Presentation: Heidelberg, Germany
• [Presentation] Translation termination factor eRF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apotosis (2012)
  • Author(s): Yoshifumi Hashimoto, Nao Hosoda, Pinaki Datta, Emad S. Alnemri, and Shin-ichi Hoshino
  • Organizer: The Complex Life of mRNA, EMBO Symposia
  • Place of Presentation: Heidelberg, Germany
• [Presentation] 癌抑制遺伝子産物Tobによる転写後のc-myc遺伝子発現調節機構 (2012)
  • Author(s): 尾上耕一、細田直、船越祐司、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 静岡
• [Presentation] Translation termination factor eRF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apotosis. (2012)
  • Author(s): Yoshifumi Hashimoto, Nao Hosoda, Pinaki Datta, Emad S. Alnemri, and Shin-ichi Hoshino
  • Organizer: The Complex Life of mRNA, EMBO Symposia
  • Place of Presentation: Heidelberg, Germany
• [Presentation] B型肝炎治療を目指したmRNAとランスフェクションによる高効率遺伝子発現系の確立
  • Author(s): 野木森 拓人、細田 直、星野 真一
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会
  • Place of Presentation: 鈴鹿
• [Presentation] eRF3 ファミリーに属する G タンパク質 GTPBP1 の機能解析
  • Author(s): 西浦 久達、細田 直、星野 真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] ポリ A 鎖分解酵素 Nocturnin による mRNA 動態制御
  • Author(s): 奥村 真由、細田 直、星野 真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] 癌抑制遺伝子産物 Tob による転写後の c-myc 遺伝子発現調節機構
  • Author(s): 尾上 耕一 、細田 直、船越 祐司、星野 真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 静岡
• [Remarks]
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/syk/index.html