| Project/Area Number |
24770139
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
IMOTO Hitomi 独立行政法人国立循環器病研究センター, 研究所, 非常勤研究員 (50532230)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | NDRG4 / 神経細胞 / ナトリウム/カリウムATPase / 脳 / 心臓 / ナトリウム/カリウム-ATPase |
|---|
| Research Abstract |
NDRG4 localizes in neurons and retains the levels of BDNF in the mouse cerebral cortex, which is essential for maintaining the ability of spatial learning and neuronal protection after the temporary cerebral ischemia. NDRG4 plays important roles in the normal heart rhythm and physiological adaptation of cardiac muscle after forced swimming stress. In this study, we identified Na+/K+-ATPase (NKA) alpha3 subunit as NDRG4-interacting protein in the brain using affinity chromatography. We found that other NDRG members, NDRG1-NDRG3, also associate with NKA alpha subunit in HeLa cells cotransfected with NDRG members and NKA alpha subunit. These data indicate that NDRG members may regulate the NKA functions of ion pumping and cell signaling by interacting with NKA alpha subunit in a cell-specific manner.
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