Reversible photo-regulation of the motor functions of kinesin by a novel photochromic ATP analog
| Project/Area Number |
24770140
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
KAMEI Takashi 北海道大学, 電子科学研究所, 助教 (90450650)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | モータータンパク質 / ATPアナログ / フォトクロミック分子 |
|---|
| Research Abstract |
We obtained the following two main results in this research.
(1) We synthesized novel adenosine triphosphate (ATP) analogs, ATP-Azos, which were chimeric molecules of ATP and azobenzene compounds exhibiting a reversible and large conformational change by photo-irradiations. In aqueous solutions, photo-responsive properties of the ATP-Azos were revealed with spectroscopic methods.
(2) In the presence of an ATP-Azo, we assessed the motile properties of kinesin, a bio-molecular motor, which derives the mechanical energy from ATP hydrolysis, with or without photo-irradiations. Consequently, we realized reversible photo-modulation of motility of kinesin by irradiations.
|
Report
(3 results)
Research Products
(23 results)
