| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Bacteria can move by rotating flagella. The flagellar filament is linked with the rotary nanomachine called the flagellar motor, which is embedded in the cytoplasmic membrane. The flagellar motor consists of a rotor and stators. It is known that the proton translocation through the stator unit is converted to the mechanical work, but the energy-conversion mechanism still remains unclear. In this study, we performed a single-molecule measurement of the bacterial flagellar motor in order to elucidate the mechanism of energy conversion. Observation of a 100-nm gold nanoparticle adhered to a single flagellar filament of Salmonella showed step motions, tracks with moving and waiting periods. The step event is believed to be an elementary process of the energy-conversion reaction. Thus, our results offer important insight into the rotation mechanism of flagellar motors.
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