Non-equilibrium statistical mechanics for fluctuations in cells

• Project/Area Number: 24770143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: HAYASHI Kumiko 東北大学, 工学(系)研究科(研究院), 助教 (00585979)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 1分子計測・操作 / モータータンパク質 / 非平衡統計力学 / 揺動散逸定理 / 揺らぎの定理 / キネシン / ダイニン / 重心揺らぎ / 蛍光観察 / 生体モーター

Research Abstract

We measured the motion of mitochondria in PC12 cells by using fluorescent observation. A mitochondrion is transported by motor proteins such as kinesin and dynein along microtubules. We investigated the fluctuation-dissipation theorem for this non-equilibrium phenomenon. Comparing motion of Brownian beads incorporated into the cells by electroporation method, we experimentally found that the fluctuation-dissipation theorem was not valid for the motion of a mitochondrion.

Research Products

• [Journal Article] Viscosity and drag force involved in organelle transport : Investigation of the fluctuation dissipation theorem (2013)
  • Author(s): K. Hayashi, C.G. Pack, M. K. Sato, K. Mouri, K. Kaizu, K. Takahashi and Y. Okada
  • Journal Title: Euro Phys J E Volume: 36 Issue: 12 Pages: 136-136
  • DOI: 10.1140/epje/i2013-13136-6
  • Peer Reviewed
• [Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase (2013)
  • Author(s): Watanabe R, Hayashi K, Ueno H and Noji H
  • Journal Title: Biophys. J Volume: 105 Issue: 10 Pages: 2385-2391
  • DOI: 10.1016/j.bpj.2013.09.050
  • Peer Reviewed
• [Journal Article] Protein motor F_1 as a model system for fluctuation theories of non-equilibrium statistical mechanics (2012)
  • Author(s): K. Hayashi and R. Hayashi
  • Journal Title: Fluct. and Noise Lett. Volume: 11 Issue: 03 Pages: 124001-124001
  • DOI: 10.1142/s0219477512410015
  • Peer Reviewed
• [Journal Article] Single-molecule stochastic resonance (2012)
  • Author(s): K. Hayashi, S. de Lorenzo, M. Manosas, J. M. Huguet and F. Ritort
  • Journal Title: Phys. Rev. X Volume: 2 Issue: 3 Pages: 31012-31012
  • DOI: 10.1103/physrevx.2.031012
  • Peer Reviewed
• [Journal Article] Measurements of the driving forces of bio-motors using the fluctuation theorem (2012)
  • Author(s): K. Hayashi, M. Tanigawara and J. Kishikawa
  • Journal Title: BIOPHYSICS Volume: 8 Issue: 0 Pages: 67-72
  • DOI: 10.2142/biophysics.8.67
  • NAID: 130002061594
  • ISSN: 1349-2942
  • Peer Reviewed
• [Journal Article] Non-equilibrium Statistical Mechanics for Fluctuations in a Cell (2012)
  • Author(s): 林久美子
  • Journal Title: Seibutsu Butsuri Volume: 52 Issue: 2 Pages: 118-119
  • DOI: 10.2142/biophys.52.118
  • NAID: 10030134786
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Presentation] F-subunit reinforces torque generation in V-ATPase (2013)
  • Author(s): J. Kishikawa, A. Seino, A. Nakanishi, N. E. Tirtom, H. Noji, K. Yokoyama, K. Hayashi
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2013-10-30
• [Presentation] Transport of beads by kinesin in highly viscous environment (2013)
  • Author(s): N. Sawairi, T. Ariga, M. Tomishige, K. Hayashi
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2013-10-29
• [Presentation] Measuring the numbers of kinesin and dynein on neuronal cargo transport by using the fluctuation theorem (2013)
  • Author(s): K. Hayashi, Y. Okada
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2013-10-29
• [Presentation] Giant Acceleration of diffusion in F_1-ATPase (2013)
  • Author(s): R. Hayashi, S. Nakamura, S. Kudo, K. Sasaki, H. Noji, K. Hayashi
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2013-10-23
• [Presentation] Giant Acceleration of diffusion in F1-ATPase (2013)
  • Author(s): Ryunosuke Hayashi, Shuichi Nakamura, Seishi Kudo, Kazuo Sasaki, Hiroyuki Noji, Kumiko Hayashi
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
• [Presentation] Estimation of driving force acting on a mitochondrion transported in a living cell : Application of the fluctuation theorem (2012)
  • Author(s): K. Hayashi, M. K. Sato, K. Mouri, C. G. Pack, K. Kaizu, K. Takahashi, Y. Okada
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-23
• [Presentation] Application of new step finding algorithm to F_1-ATPase (2012)
  • Author(s): Seino, T. Nakagawa, K. Sasaki, K. Hayashi
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] Giant acceleration of diffusion in single-molecule experiments (2012)
  • Author(s): R. Hayashi, S. Nakamura, S. Kudo, K. Sasaki, H. Noji, K. Hayashi
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] Estimation of drag force acting on a mitochondrion transported in a living cell:Application of the fluctuation threom (2012)
  • Author(s): Kumiko Hayashi
  • Organizer: 日本生物物理学会第５０回年会
  • Place of Presentation: 名古屋
  • Invited
• [Book] 300号記念特集「生物物理の未来に寄せて」--非平衡統計力学による細胞内ゆらぎ測定 (2012)
  • Author(s): 林久美子
  • Total Pages: 118
  • Publisher: 生物物理