Multiscale motion analysis of membrane proteins at neuromuscular junction
Project/Area Number |
24770147
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Japan Synchrotron Radiation Research Institute |
Principal Investigator |
SEKIGUCHI Hiroshi 公益財団法人高輝度光科学研究センター, 利用研究促進部門, 研究員 (00401563)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | 1分子計測 / ナノ結晶 / 分子内運動 / 構造変化 / 揺らぎ / 1分子計測 / ダイナミクス計測 / 膜タンパク質 |
Research Abstract |
In this project, we engaged in developing the experimental technique to track internal motion and translational motion of membrane proteins at multi-time scale based on diffracted X-ray tracking method (DXT). In DXT, the internal motion of an individual single protein is monitored through the trajectory of the Laue spot from the nanocrystal labeled on the objective protein immobilized on the substrate surface. We improved a quality of the gold nanocrystal and optimized the experimental system to monitor ligand-induced motion of membrane protein, acetylcholine receptors, at several 10 micro seconds scale. Moreover, we examined a quantum dot as a DXT probe, that could be suited to track internal motion (DXT) and translational motion (fluorescence microscope) of membrane proteins.
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Report
(3 results)
Research Products
(23 results)
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[Presentation] Cooperative Motion of a Multi-subunit Protein Visualized by X-ray Single Molecule Tracking2013
Author(s)
H. Sekiguchi, Y. Yamamoto, A. Nakagawa, K. Moriya, M. Arita, K. Ichiyanagi, M. Yohda, N. Yagi and Y. C. Sasaki
Organizer
57th Annual Meeting of Biophysical Society
Place of Presentation
Pennsylvania Convention Center, Philadelphia, PA, USA
Related Report
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[Presentation] 3D Micro-second X-ray Single Molecule Tracking of Nicotinic Acetylcholine Receptor with Picometer Accuracy2013
Author(s)
M. Tokue, K .Hoshisashi, H. Sekiguchi, N. Yagi, K. Ichiyanagi, Y. Nishino, A. Miyazawa, T. Kubo, Y. C. Sasaki
Organizer
57th Annual Meeting of Biophysical Society
Place of Presentation
Pennsylvania Convention Center, Philadelphia, PA, USA
Related Report
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[Presentation] 複合体タンパク質・分子内運動の高速1分子計測2012
Author(s)
関口博史, 山本陽平, 有田真優乃, 西野有里, 宮澤淳夫, 養王田正文, 八木直人, 佐々木裕次
Organizer
新学術領域「揺らぎと生体機能」「水和とATP」合同公開シンポジウム「ゆらぎと水−生命のエネルギーと機能の分子機構を探る」
Place of Presentation
大阪ガーデンパレス
Related Report
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[Presentation] Cooperativity analysis of multi-subunit proteins by 3D X-ray single molecule tracking2012
Author(s)
H. Sekiguchi, Y. Yamamoto, M. Ariga, Y. Nishino, K. Ichiyanagi, N. Yagi, A. Miyazawa, M. Yohda, Y. C. Sasaki
Organizer
International workshop on 3D atomic imaging at nano-scale active sites in materials (3D-AINAS 2012)
Place of Presentation
Media hall in Kashiwa library, University of Tokyo, Chiba, JAPAN
Related Report
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