Chemo-mechanical coupling mechanism of FoF1-ATP synthase
| Project/Area Number |
24770148
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WATANABE Rikiya 東京大学, 工学(系)研究科(研究院), 助教 (30540108)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 1分子生物物理学 / ATP合成酵素 |
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| Research Abstract |
In this project, for understanding of the chemo-mechanical coupling mechanism of FoF1-ATP synthase, I attempted to visualize the rotary motion of FoF1 at single molecule level. In 2012, I succeeded to directly visualize the proton-driven rotation of FoF1 at single molecule level (Watanabe et al., Nat Commun 2013). In 2013, I developed the novel platform to detect the proton pump activity of FoF1 at single molecule level (Watanabe et ala., submitted). For further understanding of FoF1, the method developed in this project is promising for the future.
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force2013
Author(s)
Watanabe, R., Tabata, KV., Iino, R., Ueno, H., Iwamoto, M., Oiki, S., & Noji, H
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Journal Title
Nature Communications
Volume: 4
Pages: 1631-1631
Related Report
Peer Reviewed
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[Journal Article] Torque generation mechanism of F1-ATPase upon NTP binding
Author(s)
Arai, HC., Yukawa, A., Iwatate, RJ., Kamiya, M., Watanabe, R., Urano, Y., & Noji, H.
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Journal Title
Biophysical Journal
Volume: (in press)
Related Report
Peer Reviewed
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[Presentation] High throughput formation of sub-million lipid membrane arrays for measuring membrane protein activities2013
Author(s)
Watanabe, R., Fujita, D., Tabata, KV., Yamauchi, L., Soga, N., Kim, SH., Suga, H., & Noji, H.
Organizer
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS2013)
Place of Presentation
フライブルグ、ドイツ
Year and Date
2013-10-28
Related Report
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[Presentation] High throughput formation of sub-million lipid membrane arrays for measuring membrane protein activities
Author(s)
Watanabe, R., Fujita, D., Tabata, KV., Yamauchi, L., Soga, N., Kim, SH., Suga, H & Noji, H.
Organizer
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Presentation
Freiburg, Germany
Related Report
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[Presentation] FoF1-ATP合成酵素のプロトン駆動力による回転運動の直接観察
Author(s)
Watanabe, R., Okuno, D., Iino, R., Shimabukuro, K., Yoshida, M. & Noji, H.
Organizer
日本生体エネルギー研究会
Place of Presentation
岡山・日本
Related Report
Invited
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