| Project/Area Number |
24770151
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
TATSUYA Iwata 名古屋工業大学, 工学(系)研究科(研究院), 助教 (20569917)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | フラビン / DNA修復 / 光回復酵素 / シクロブタン型ピリミジン二量体 / (6-4)光産物 / 光産物 / シクロブタン型ピリミジンダイマー |
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| Research Abstract |
I carried out the following research experiments for the elucidation of reaction mechanism of DNA photolyases. (1) I measured the detailed photoreaction of Xenopus (6-4) photolyase by UV-vis and FTIR spectroscopy. (2) I measured light induced difference FTIR spectra which accompany the structural changes of photoactivation and photorepair processes of E.coli CPD photolyase by FTIR spectroscopy, and compared with those of Xenopus (6-4) photolyase.
I obtained the following results. (1) I succeeded in the detection of the structural change accompanying a series of responsives of the photoreaction of FAD, "oxidized from - neutral semiquinoid form - reduced form" at 277 K. I was also able to check the accumulation of the anionic radical form illuminated at 200 K. (2) Upon the CPD binding to CPD photolyase, the structural changes of the alpha helices take place. The style of the structural changes was reverse compared to those of (6-4) photolyase.
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