| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
We investigated the compartment size dependency of the protein synthesis reaction using quartz-glass microchambers. By introducing the glass chamber, the concentration of the synthesized protein was significantly improved compared to that in the poly(dimethylsiloxane) (PDMS) microchamber. The concentration was below the detection limit in the PDMS chambers, whereas the glass chambers yielded 41% of the bulk reaction. When the cell-free protein synthesis reaction with different reaction orders was conducted in glass chambers, the reaction dynamics was largely affected by the chamber size although the synthesis reaction was initiated from a constant copy number of DNA. We were able to observe the properties specific to the high order reaction and found the presence of an optimum compartment volume for a high-order reaction using real biological molecules.
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