small RNA mediated DNA methylation

Research Project

Project/Area Number	24770167
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Molecular biology
Research Institution	Osaka University
Principal Investigator	IPPEI Nagamori 大阪大学, 医学(系)研究科(研究院), 助教 (20624729)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	DNAメチル化 / PIWI / MILI / MIWI2 / piRNA / レトロトランスポゾン / 核膜
Research Abstract	Retrotransposon occupies approximately 40% of genome DNA, and it is widely known that DNA methylation mediated retrotransposon repressions are critical for mammal. During male germ cell differentiation, DNA methylation is globally erased, followed by obtaining DNA methylation at site specific manner, including Retrotransposon. Since DNA methylation causes gene repression, site specificity of DNA methylation is critical, however, it remains elusive how site specificity of DNA methylation is determined. By genetic analysis, Mili and Miwi2 were identified as important genes for to determine site specificity for DNA methylation, however, the precise molecular mechanisms are largely unknown. In this project, I have reported how MILI and MIWI2 contribute to site specificity of DNA methylation for retrotransposon