| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Satellite I RNA is noncoding RNA transcribed from human centromeric repetitive sequence. Depletion of this RNA caused the abnormal nuclear morphology due to defects in chromosome segregation. We founded that Aurora kinase B, a key factor of chromosome segregation binds to this RNA. Aurora B was regulated kinase activity and localization through binding to this RNA. To identify interaction factors to satellite I RNA, we performed pulled down of Satellite I RNA. Several interactors were identified from pulled down products by LC/MS analysis. Depletion of RBMX, one of interaction protein, showed phenotype of defect in chromosome segregation.
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