| Project/Area Number |
24770169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kochi University of Technology |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHAMA Takeshi 高知工科大学, 環境理工学群, 教授 (00194267)
CERUTTI Heriberto ネブラスカ大学リンカーン校, プラントサイエンスイニシアチブ, 教授
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マイクロRNA / RNAサイレンシング / クラミドモナス / 分子生物学 / microRNA |
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| Research Abstract |
A gene exerts its function in a cell, and the gene expression is strictly controlled by diverse regulation systems. Small non-cording RNA, which is called miRNA, bind to specific mRNA with their complementarity and induce gene silencing. Gene silencing is mediated by cleavage of a target mRNA or translation inhibition of a target mRNA. Previously, we found that artificial miRNA regulates gene expression at translation levels in unicellular geen alga Chlamydomonas reinhatdtii. Therefore, some endogenous genes were suspected to be regulated by miRNAs at translation levels, but it has not been demonstrated. In this research, we tried to find miRNA that mediate translation repression, and we found that a miRNA inhibits translation of a protein kinase in Chlamydomonas.
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