| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Centromere is essential structure for accurate chromosome segregation in mitosis and meiosis. To elucidate the molecular mechanism maintaining the function of a centromere, we have developed the convenient and advantageous artificially inducible system fusing two chromosomes with the terminal in fission yeast. Screening of deletion mutants by this system indicated that eight strains increased the frequencies of survivors when fused chromosome was generated and two centromeres were harbored in one chromosome. In these mutant, many of survivors harbored a fused chromosome in which either centromere was epigenetically inactivated. These results suggested that the centromeres tend to inactivate in these gene deletions compared to a wild type. Because these genes encoded the proteins related to modification of histone, specific histone modification may be important for inhibition of centromere inactivation.
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