Molecular mechanisms for converting meiosis I to meiosis II
| Project/Area Number |
24770173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
KITAJIMA Tomoya 独立行政法人理化学研究所, 発生・再生科学総合研究センター, チームリーダー (00376641)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 卵母細胞 / ライブイメージング / 動原体 / 染色体動態 / 減数分裂 / 染色体 |
|---|
| Research Abstract |
Cohesion between sister kinetochores is known to be released during the transition from meiosis I to meiosis II. This study aimed to reveal molecular mechanisms for the release of sister kinetochore cohesion. Live imaging of kinetochores at high resolution in living mouse oocytes indicated that sister kinetochores separated at the onset of anaphase I. Moreover, a newly developed biosensor detected a high activity of separase which cleaves Rec8 cohesin. Thus, these data support the idea that the release of sister kinetochore cohesion is mediated by cleavage of Rec8 by separase.
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Report
(3 results)
Research Products
(28 results)
