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Elucidation of the spatio-temporal regulated formation mechanism of cellular actin-based structures.

Research Project

Project/Area Number 24770175
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionTohoku University

Principal Investigator

MIZUNO Hiroaki  東北大学, 生命科学研究科, 助教 (00620204)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords細胞骨格 / アクチン / フォルミン / コフィリン / 螺旋回転 / 螺旋構造
Research Abstract

In cells, the formation of actin structures is regulated by the interaction between actin regulatory proteins. Formin homology proteins (formins) play an essential role in the formation of actin stress fibers and yeast actin cables. A mammalian formin mDia1 rotates along the long-pitch helix of F-actin during processive actin elongation (helical rotation). Helical rotation may impose torsional force on F-actin in the opposite direction to the twisting of F-actin by an actin depolymerizing factor cofilin. In the present study, we revealed that helical rotation of mDia1 imposes torsional force on F-actin and enhances resistant against the cofilin binding and the filament severing activity of cofilin. In addition, an active mutant of mDia1 tethered to cellular structures through its N-terminal region induces the formation of F-actin resistant to actin disassembly. These results indicate that helical rotation of mDia1 functions as an antagonistic mechanism to the cofilin activity.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (4 results) (of which Invited: 2 results)

  • [Journal Article] Rotational movement of formins evaluated by using single-molecule fluorescence polarization2014

    • Author(s)
      Mizuno, H. and Watanabe, N.
    • Journal Title

      Methods Enzymol

      Volume: 540 Pages: 73-94

    • DOI

      10.1016/b978-0-12-397924-7.00005-4

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales.2014

    • Author(s)
      Yamashiro S, Mizuno H, Smith MB, Ryan GL, Kiuchi T, Vavylonis D, Watanabe N
    • Journal Title

      Molecular Biology of the Cell

      Volume: 25 Issue: 7 Pages: 1010-1024

    • DOI

      10.1091/mbc.e13-03-0162

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] F and G-actin homeostasis regulates mechanosensitive actin nucleation by formins2013

    • Author(s)
      Higashida C, Kiuchi T, Akiba Y, Mizuno H, Maruoka M, Narumiya S, Mizuno K, Watanabe N
    • Journal Title

      Nature Cell Biol

      Volume: 15(4) Issue: 4 Pages: 395-405

    • DOI

      10.1038/ncb2693

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] mDia1 and formins: screw cap of the actin filament2012

    • Author(s)
      Mizuno, H. and Watanabe, N.
    • Journal Title

      BIOPHYSICS

      Volume: 8 Issue: 0 Pages: 95-102

    • DOI

      10.2142/biophysics.8.95

    • NAID

      130002061590

    • ISSN
      1349-2942
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] フォルミンタンパク質のアクチン二重螺旋に沿った回転重合2012

    • Author(s)
      渡邊直樹,水野裕昭
    • Journal Title

      化学と生物

      Volume: 第50巻第11号

    • URL

      https://www.jstage.jst.go.jp/article/kagakutoseibutsu/50/11/50_801/_pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] アクチン研究の最新動向―構造から調節,恒常性,可視化,モデリングまで2012

    • Author(s)
      山城佐和子、圓岡真宏、水野裕昭、渡邊直樹
    • Journal Title

      実験医学

      Volume: 第30巻第18号 Pages: 2998-3005

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 一分子蛍光偏光を用いたアクチン線維の螺旋構造に沿ったフォルミンの回転運動の可視化2013

    • Author(s)
      水野裕昭、山城佐和子、渡邊直樹
    • Organizer
      日本顕微鏡学会第57回シンポジウム
    • Place of Presentation
      名古屋 (口頭)
    • Year and Date
      2013-11-16
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 一分子蛍光偏光を用いたアクチン線維の螺旋構造に沿ったフォルミンの回転運動の可視化2013

    • Author(s)
      水野 裕昭
    • Organizer
      日本顕微鏡学会第57回シンポジウム
    • Place of Presentation
      愛知県産業労働センター(ウインクあいち)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Actin filament stabilization by helical rotation of mammalian formin mDial2012

    • Author(s)
      水野裕昭、山城佐和子、渡邊直樹
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡 (口頭)
    • Year and Date
      2012-12-12
    • Related Report
      2013 Final Research Report
  • [Presentation] フォルミンmDia1の螺旋回転を介したアクチン脱重合抵抗性2012

    • Author(s)
      水野裕昭
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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