| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Cytokinesis is essential for proliferation of any type of cell. Especially in animal cells and fungi, the contractile ring --a circular structure that is composed of actin filaments and myosin-II like muscle sarcomere-- plays an important role in the spatiotemporally regulated cytofission. In this study, we aimed to elucidate the molecular basis of actin-myosin-II interaction in the contractile ring. We found that an actin-binding protein that localized to the ring and is vital for its assembly, Rng2, regulated ATPase and motor activities of myosin-II and that myosin-II was involved in maintenance of the ring at later anaphase, not only in its assembly and constriction. These findings may greatly contribute to a comprehensive understanding of the whole mechanism of cytokinesis at molecular level and to medical applications, for example development of a technique for regulating growth of some type of cell such as cancer cells.
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