| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Autophagy is a conserved degradation process, wherein autophagosomes are generated by cooperative actions of multiple autophagy-related (Atg) proteins. Previous studies using yeast Saccharomyces cerevisiae have provided various insights into the molecular basis of autophagy, however, the most intriguing issues in autophagy, namely, the origin of the autophagosomal membranes, remain to be elucidated. In this study, we succeeded to purify the autophagosomal membranes and the Atg9 vesicles, one of the autophagy-related membranes, and subjected them to proteomic analyses. These analyses showed that both the Atg9 vesicles and the autophagosomal membranes contain the TRAPPIII complex and its downstream factor Ypt1. Furthermore, we found that the Atg9 vesicles become part of the autophagosomal membranes, thereby recruiting the TRAPPIII complex and Ypt1 onto the autophagosomal membranes.
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