Proteomic analyses of autophagy-related structures in yeast
Project/Area Number |
24770182
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Cell biology
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
YAMAMOTO Hayashi 東京工業大学, フロンティア研究機構, 特任助教 (80551283)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | オートファジー / オートファゴソーム / 出芽酵母 / 膜動態 / 膜小胞 / プロテオーム解析 / ベシクル |
Research Abstract |
Autophagy is a conserved degradation process, wherein autophagosomes are generated by cooperative actions of multiple autophagy-related (Atg) proteins. Previous studies using yeast Saccharomyces cerevisiae have provided various insights into the molecular basis of autophagy, however, the most intriguing issues in autophagy, namely, the origin of the autophagosomal membranes, remain to be elucidated. In this study, we succeeded to purify the autophagosomal membranes and the Atg9 vesicles, one of the autophagy-related membranes, and subjected them to proteomic analyses. These analyses showed that both the Atg9 vesicles and the autophagosomal membranes contain the TRAPPIII complex and its downstream factor Ypt1. Furthermore, we found that the Atg9 vesicles become part of the autophagosomal membranes, thereby recruiting the TRAPPIII complex and Ypt1 onto the autophagosomal membranes.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Atg9 vesicles recruit vesicle- tethering proteins Trs85 and Ypt1 to the autophagosome formation site2012
Author(s)
Kakuta, S., Yamamoto, H., Negishi, L., Kondo-Kakuta, C., Hayashi, N., and Ohsumi, Y.
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Journal Title
J. Biol. Chem.
Volume: Vol.287
Issue: 53
Pages: 44261-44269
DOI
Related Report
Peer Reviewed
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[Journal Article] Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7.2012
Author(s)
Yamaguchi M, Matoba K, Sawada R, Fujioka Y, Nakatogawa H, Yamamoto H, Kobashigawa Y, Hoshida H, Akada R, Ohsumi Y, Noda NN, Inagaki F.
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 19
Issue: 12
Pages: 1250-1256
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate2012
Author(s)
Yamaguchi, M., Noda, N. N., Yamamoto, H., Shima, T., Kumeta, H., Kobashigawa, Y., Akada, R., Ohsumi, Y. and Inagaki, F
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Journal Title
Structure
Volume: Vol.20
Issue: 7
Pages: 1244-1254
DOI
Related Report
Peer Reviewed
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