Molecula mechanism of mRNA targeting to the ER in SRP-independent manner
| Project/Area Number |
24770185
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
YOSHIDA Hideki 京都工芸繊維大学, 工芸科学研究科, 助教 (30570600)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | RNAイメージング / mRNAの小胞体標的化 / ショウジョウバエ / 培養細胞 / mRNAの局在化 / 小胞体標的化 / mRNA |
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| Research Abstract |
To reveal the novel mechanism of mRNA targeting to the ER, we had established green fluorescent protein (GFP)-mediated RNA visualization system in Drosophila cultured cells to analyze the mRNA localization and widely identified the mRNAs which are targeted to the ER in a novel manner. As a result of the visualization of mRNA localization, which is targeted in a conventional mechanism by using the system we established, GFP dot signals were observed in the cytoplasm. On the other hand, the GFP signals were hardly observed in the conventional targeting mechanism-disrupted cells. From these data, we have judged the visualization system we established certainly works. By this visualization system, the localization of mRNA, which was identified as a mRNA targeted to the ER in non-conventional manner, was observed and the mRNA also formed foci in the cytoplasm. Interestingly, the GFP dot formation was not affected even if the conventional mechanism was suppressed by RNA interference.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] Drosophila MCM10 is required for DNA replication and differentiation in the compound eye.2014
Author(s)
Vo, N., Taga, A., Inaba, Y., Hideki, Y., Cotterill, S. and Yamaguchi, M.
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Journal Title
PLoS ONE
Volume: 9
Issue: 3
Pages: 1-15
DOI
Related Report
Peer Reviewed
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[Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.2014
Author(s)
Yanai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
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Journal Title
Oncogenesis
Volume: 3
Issue: 4
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.2012
Author(s)
Ueda T., Kobori, A., Yamayoshi, A., Yoshida, H., Yamaguchi, M. , Murakami, A.
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Journal Title
Bioorganic & Medicinal Chemistry
Volume: 20
Issue: 20
Pages: 6034-6039
DOI
Related Report
Peer Reviewed
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[Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle.2012
Author(s)
Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K. and Yamaguchi, M.
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Journal Title
Gene
Volume: 495 (2),
Issue: 2
Pages: 104-114
DOI
Related Report
Peer Reviewed
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[Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis.2012
Author(s)
Ushijima Y., Inoue, Y. H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H. and Yamaguchi, M.
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Journal Title
Chromosome Res.
Volume: 20
Pages: 319-331
Related Report
Peer Reviewed
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