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Molecula mechanism of mRNA targeting to the ER in SRP-independent manner

Research Project

Project/Area Number 24770185
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionKyoto Institute of Technology

Principal Investigator

YOSHIDA Hideki  京都工芸繊維大学, 工芸科学研究科, 助教 (30570600)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
KeywordsRNAイメージング / mRNAの小胞体標的化 / ショウジョウバエ / 培養細胞 / mRNAの局在化 / 小胞体標的化 / mRNA
Research Abstract

To reveal the novel mechanism of mRNA targeting to the ER, we had established green fluorescent protein (GFP)-mediated RNA visualization system in Drosophila cultured cells to analyze the mRNA localization and widely identified the mRNAs which are targeted to the ER in a novel manner. As a result of the visualization of mRNA localization, which is targeted in a conventional mechanism by using the system we established, GFP dot signals were observed in the cytoplasm. On the other hand, the GFP signals were hardly observed in the conventional targeting mechanism-disrupted cells. From these data, we have judged the visualization system we established certainly works. By this visualization system, the localization of mRNA, which was identified as a mRNA targeted to the ER in non-conventional manner, was observed and the mRNA also formed foci in the cytoplasm. Interestingly, the GFP dot formation was not affected even if the conventional mechanism was suppressed by RNA interference.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (45 results)

All 2014 2013 2012

All Journal Article (20 results) (of which Peer Reviewed: 20 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (25 results)

  • [Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling phathway2014

    • Author(s)
      Hiroshi Yanai
    • Journal Title

      Ongogenesis

      Volume: 3 Pages: 1-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye2014

    • Author(s)
      Nicole Vo
    • Journal Title

      PLOS ONE

      Volume: 9 Pages: 1-15

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila MCM10 is required for DNA replication and differentiation in the compound eye.2014

    • Author(s)
      Vo, N., Taga, A., Inaba, Y., Hideki, Y., Cotterill, S. and Yamaguchi, M.
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 3 Pages: 1-15

    • DOI

      10.1371/journal.pone.0093450

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.2014

    • Author(s)
      Yanai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
    • Journal Title

      Oncogenesis

      Volume: 3 Issue: 4 Pages: 1-7

    • DOI

      10.1038/oncsis.2014.13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional analysis of Drosophila DNA polymerase e p58 subunit2013

    • Author(s)
      Ritsuko Sahashi
    • Journal Title

      Am. J. Cancer Res.

      Volume: 3 Pages: 478-489

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells2013

    • Author(s)
      Koichi Eguchi
    • Journal Title

      Exp. Cell Res.

      Volume: 319 Pages: 2179-2195

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development2013

    • Author(s)
      Ly Luong Linh
    • Journal Title

      Gene

      Volume: 520 Pages: 106-118

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DREF, a concertmaster for Hippo pathway and JNK pathway in Drosophila.2013

    • Author(s)
      Fujiwara, S., Yoshida, H. and Yamaguchi, M.
    • Journal Title

      J. Carcinogene. Mutagene.

      Volume: 4 Issue: 02

    • DOI

      10.4172/2157-2518.1000e110

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of Drosophila RFX3 induces apoptosis and interferes with differentiation of photoreceptor cells.2013

    • Author(s)
      Sugimoto, T., Ueda, T., Yoshida, H., Murakami, A. and Yamaguchi, M.
    • Journal Title

      J. Carcinogene. Mutagene.

      Volume: 4(1) Issue: 02 Pages: 1-7

    • DOI

      10.4172/2157-2518.1000137

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development.2013

    • Author(s)
      Luong Linh Ly, Osamu Suyari, Yasuhide Yoshioka, Nguyen Trong Tue, Hideki Yoshida, Masamitsu Yamaguchi
    • Journal Title

      Gene

      Volume: 520 Pages: 106-118

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells.2013

    • Author(s)
      Koichi Eguchi, Yasuhide Yoshioka, Hideki Yoshida, Kazushige Morishita, Seiji Miyata, Hiroshi Hiai, Masamitsu Yamaguchi
    • Journal Title

      Exp. Cell Res.

      Volume: 319 Pages: 2179-2195

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of Drosophila DNA polymerase ε p58 subunit.2013

    • Author(s)
      Ritsuko Sahashi, Risa Matsuda, Osamu Suyari, Mieko Kawai, Hideki Yoshida, Sue Cotterill, Masamitsu Yamaguchi
    • Journal Title

      Am. J. Cancer Res.

      Volume: 3 Pages: 478-489

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DREF, a concertmaster for Hippo pathway and JNK pathway in Drosophila.2013

    • Author(s)
      Fujiwara, S., Yoshida, H. and Yamaguchi, M.
    • Journal Title

      J. Carcinogene. Mutagene.

      Volume: 4

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.2012

    • Author(s)
      Ueda T., Kobori, A., Yamayoshi, A., Yoshida, H., Yamaguchi, M. , Murakami, A.
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 20 Issue: 20 Pages: 6034-6039

    • DOI

      10.1016/j.bmc.2012.08.028

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sarcoglycan complex formation is involved in regulation of EGFR signaling during Drosophila eye development.2012

    • Author(s)
      Hashimoto, R., Yoshida, H. and Yamaguchi, M.
    • Journal Title

      HOAJ Biology,

      Volume: 1(1) Issue: 1 Pages: 1-1

    • DOI

      10.7243/2050-0874-1-7

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis2012

    • Author(s)
      Ushijima Y, Inoue YH, Konishi T, Kitazawa D, Yoshida H, Shimaji K, Kimura H, Yamaguchi M
    • Journal Title

      Chromosome Research

      Volume: 3 Issue: 3 Pages: 319-31

    • DOI

      10.1007/s10577-012-9276-1

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle.2012

    • Author(s)
      Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K. and Yamaguchi, M.
    • Journal Title

      Gene

      Volume: 495 (2), Issue: 2 Pages: 104-114

    • DOI

      10.1016/j.gene.2011.12.056

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway2012

    • Author(s)
      Shunsuke Fujiwara
    • Journal Title

      Am. J. Cancer Res.

      Volume: 2 Pages: 36-44

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway.2012

    • Author(s)
      Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
    • Journal Title

      Am. J. Cancer Res.

      Volume: 2 Pages: 36-44

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis.2012

    • Author(s)
      Ushijima Y., Inoue, Y. H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H. and Yamaguchi, M.
    • Journal Title

      Chromosome Res.

      Volume: 20 Pages: 319-331

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of non-canonical mRNAs targeting to the ER and analysis on subcellular localization of mRNA by RNA imaging system2014

    • Author(s)
      梅河内隆成
    • Organizer
      The 19^<th> Annual meeting of RNA Society
    • Place of Presentation
      カナダ
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエ初期胚を用いたmetaphase furrow様局在を示すmRNAの局在解析2014

    • Author(s)
      田中領
    • Organizer
      第3回 サイエンスインカレ発表会
    • Place of Presentation
      幕張メッセ国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of non-canonical mRNAs targeting to the ER and analysis on subcellular localization of mRNAs by RNA imaging system2014

    • Author(s)
      Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tetsuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Hideki Yoshida
    • Organizer
      The 19th Annual Meeting of the RNA Society
    • Place of Presentation
      Quebec City Convention Centre
    • Related Report
      2013 Annual Research Report
  • [Presentation] Localization analysis of predicted methaphase furrow-localized mRNA in Drosophila early embryos2014

    • Author(s)
      Ryo Tanaka, Krause M. Henry, Masamitsu Yamaguchi, Hideki Yoshida
    • Organizer
      The 19th Annual Meeting of the RNA Society
    • Place of Presentation
      Quebec City Convention Centre
    • Related Report
      2013 Annual Research Report
  • [Presentation] Localization analysis of predicted metaphase furrow-localized mRNA in Drosophila early embryos2013

    • Author(s)
      田中領
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of mRNAs not bearing signal sequence in the ER fraction and analysis of the mRNAs subcellular localization2013

    • Author(s)
      梅河内隆成
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] 非古典的経路で小胞体へ標的化するmRNAの同定及び同機構解析系の構築2013

    • Author(s)
      梅河内隆成
    • Organizer
      第65回日4細胞生物学会大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] 非古典的経路で小胞体へ標的化するmRNAの同定及び同機構解析系の構築2013

    • Author(s)
      梅河内隆成、越田大夢、山田百子、臼井一馬、佐藤哲也、伊藤恵美、大川恭行、山口政光、吉田英樹
    • Organizer
      第65回 日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of mRNAs not bearing signal sequence in the ER fraction, and analysis of the mRNAs subcellular localization2013

    • Author(s)
      梅河内隆成、越田大夢、山田百子、臼井一馬、佐藤哲也、須山幹太、伊藤恵美、大川恭行、山口政光、吉田英樹
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Localization analysis of predicted methaphase furrow-localized mRNA in Drosophila early embryos2013

    • Author(s)
      Ryo Tanaka, Krause Henry, Masamitsu Yamaguchi, Hideki Yoshida
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Localization analysis of predicted metaphase furrow-localized mRNA in Drosophila early embryos2012

    • Author(s)
      田中領
    • Organizer
      The 19^<th> Annual meeting of RNA Society
    • Place of Presentation
      カナダ
    • Related Report
      2013 Final Research Report
  • [Presentation] Cabezaノックダウンはショウジョウバエ成虫複眼の形態異常を誘導する2012

    • Author(s)
      島村真衣
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ショウジョウバエDNAポリメラーゼp255サブユニットと相互作用する遺伝子の探索2012

    • Author(s)
      前田頌太
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエDOCK family遺伝子の機能解析2012

    • Author(s)
      森下和茂
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] A Drosophila model for function analysis of organic carrier partner 12012

    • Author(s)
      Nguyen Huu Tho
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエ精子形成過程におけるエビジェネティック制御因子Jumonji(Jarid2)の動態2012

    • Author(s)
      戸田成美
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ショウジョウバエ転写因子DREFを中心とした転写制御ネットワーク : DREFの標的としてのHippo経路関連遺伝子の同定2012

    • Author(s)
      藤原俊介
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] mRNA新規小胞体標的化機構の分子メカニズムの解明2012

    • Author(s)
      梅河内隆成
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development2012

    • Author(s)
      Ly Luong Linh
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of mRNAs targeting to the ER2012

    • Author(s)
      梅河内隆成
    • Organizer
      The 22^<nd > CDB Meeting : RNA Sciences in cell and developmental biology II
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエDNAポリメラーゼε p255サブユニットと相互作用する遺伝子の探索2012

    • Author(s)
      前田 頌太、須鑓 理、佐橋 律子、稲葉 泰裕、吉田 英樹、山口 政光
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] ショウジョウバエ DOCK family遺伝子の機能解析2012

    • Author(s)
      森下 和茂、小笹 史登、吉田 英樹、山口 政光
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] dNF-YB Plays Dual Roles in Cell Death and Cell Differentiation of Drosophila Eye Developmental2012

    • Author(s)
      Ly Luong Linh, Tue Nguyen Trong, Osamu Suyari, Yasuhide Yoshioka, Hideki Yoshida, Masamitsu Yamaguchi
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 蛍光タンパク質を基盤とした小胞他標的化RNA可視化システムの構築2012

    • Author(s)
      越田 大夢、山田 百子、梅河内 隆成、Krause Henry、山口 政光、吉田 英樹
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Identification of mRNA targeting to the ER2012

    • Author(s)
      Umegawachi Takanari、Usui Kazuma、Sato Tetsuya、Ito Megumi、Ohkawa Yasuyuki、Suyama Mikita、Yamaguchi Masamitsu、Yoshida Hideki
    • Organizer
      The 22nd CDB Meeting RNA Sciences in cell and developmental biology II
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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