Mechanism of virtual nuclear envelope breakdown

• Project/Area Number: 24770187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: ASAKAWA Haruhiko 大阪大学, 生命機能研究科, 助教 (70399533)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 核構造 / 核膜孔複合体 / 核膜 / 分裂酵母 / 核膜孔 / 減数分裂

Research Abstract

In fission yeast, a phenomenon functionally equivalent of nuclear envelope breakdown (virtual nuclear envelope breakdown: V-NEBD) occurs in meiosis to increase nuclear envelope permeability with maintenance of nuclear envelope and nuclear pore structure. To understand the function of nuclear pore complex on the V-NEBD, about 30 proteins composing nuclear pore complex were characterized by their localizations and expression levels. These analyses suggested the unique composition of nuclear pore complex in fission yeast. In addition, gene disruption analysis of the nuclear pore complex genes revealed a gene that regulates the timing of V-NEBD.

Research Products

• [Journal Article] Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe (2014)
  • Author(s): Asakawa H, Yang HJ, Yamamoto T G, Ohtsuki C, Chikashige Y, Sakata-Sogawa K, Tokunaga M, Iwamoto M, Hiraoka Y, Haraguchi T.
  • Journal Title: Nucleus Volume: 5(印刷中) Issue: 2 Pages: 1-14
  • DOI: 10.4161/nucl.28487
  • Peer Reviewed / Open Access
• [Journal Article] A method of correlative light and electron microscopy for yeast cells (2014)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Journal Title: Micron Volume: 61 Pages: 53-61
  • DOI: 10.1016/j.micron.2014.02.007
  • Peer Reviewed / Open Access
• [Journal Article] Monoclonal antibodies recognize Gly-Leu-Phe-Gly repeat of nucleoporin Nup98 of Tetrahymena, yeasts and humans (2013)
  • Author(s): Masaaki Iwamoto et al.
  • Journal Title: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Volume: 32 Issue: 2 Pages: 81-90
  • DOI: 10.1089/mab.2012.0118
  • Peer Reviewed
• [Presentation] 染色体安定性に関わる分裂酵母核膜タンパク質 (2013)
  • Author(s): 丹下喜恵, 淺川東彦, 原口徳子, 平岡泰
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸市
  • Year and Date: 2013-12-05
• [Presentation] Nup132, a NPC scaffolding protein, mediates meiotic progression in the fission yeast Schizosaccharomyces pombe (2013)
  • Author(s): Yang H-J, Asakawa H, Haraguchi T, Hiraoka Y
  • Organizer: EMBO Conference on Meiosis
  • Place of Presentation: Hotel Radisson Blu, Radebeul-Dresden, Germany
  • Year and Date: 2013-09-17
• [Presentation] Fission yeast nuclear membrane proteins that affect chromosome movements (2013)
  • Author(s): Tange Y, Asakawa H, Yang H-J, Chikashige Y, Haraguchi T, Hiraoka Y
  • Organizer: The seventh international fission yeast meeting, EMBO Conference on Fission Yeast : pombe2013
  • Place of Presentation: Senate House, London, UK
  • Year and Date: 2013-06-29
• [Presentation] Meiotic nuclear movement in fission yeast is prolonged by stalled DNA replication through a Cds1-dependent checkpoint pathway (2013)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Haraguchi T, Masukata H, Hiraoka Y
  • Organizer: The seventh international fission yeast meeting, EMBO Conference on Fission Yeast : pombe2013
  • Place of Presentation: Senate House, London, UK
• [Presentation] GFP融合ヌクレオポリンを用いた分裂酵母核膜孔複合体の構成の解析 (2012)
  • Author(s): 淺川東彦, 大槻千鶴, 十川久美子, 徳永万喜洋, 平岡泰, 原口徳子
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会(共同開催)
  • Place of Presentation: 淡路夢舞台国際会議場,兵庫県淡路市
  • Year and Date: 2012-12-20
• [Presentation] The replication stress causes prolonged nuclear movement and chromosome decompaction in fission yeast (2012)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Hiraoka Y
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会(共同開催)
  • Place of Presentation: 淡路夢舞台国際会議場,兵庫県淡路市
  • Year and Date: 2012-12-20
• [Presentation] 減数分裂期のDNA複製の欠損は分裂酵母のhorsetail核運動時間の伸長とクロマチン構造異常をもたらす (2012)
  • Author(s): Ruan K, 山本孝治, 浅川東彦, 平岡泰
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡,福岡市
  • Year and Date: 2012-12-13
• [Presentation] Organization of the nuclear pore complex in fission yeast Schizosaccharomyces pombe (2012)
  • Author(s): Asakawa H, Chikashige Y, Ohtsuki C, Iwamono M, Hiraoka Y, Haraguchi T
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場,神戸市
  • Year and Date: 2012-05-31
• [Presentation] Biased assembly of the nuclear pore complex determines nuclear differentiation in the ciliate Tetrahymena thermophila (2012)
  • Author(s): Iwamoto M, Koujin T, Bunai F, Osakada H, Mori C, Asakawa H, Hiraoka Y, Haraguchi T
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場,神戸市
  • Year and Date: 2012-05-29
• [Presentation] GFP融合ヌクレオポリンを用いた分裂酵母核膜孔複合体の構成の解析 (2012)
  • Author(s): 淺川東彦
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Presentation] Organization of the nuclear pore complex in fission yeast Schizosaccharomyces pombe. (2012)
  • Author(s): Asakawa H
  • Organizer: 第45 回日本発生生物学会・第64 回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] The replication stress causes prolonged nuclear movement and chromosome decompaction in fission yeast. (2012)
  • Author(s): Ruan K
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Presentation] Biased assembly of the nuclear pore complex determines nuclear differentiation in the ciliate Tetrahymena thermophila. (2012)
  • Author(s): Iwamoto M
  • Organizer: 第45 回日本発生生物学会・第64 回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] 減数分裂期のDNA複製の欠損は分裂酵母のhorsetail核運動時間の伸長とクロマチン構造異常をもたらす (2012)
  • Author(s): Ruan K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] Nup132, a NPC scaffolding protein, mediates meiotic progression in the fission yeast Schizosaccharomyces pombe.
  • Author(s): Yang HJ, Asakawa H, Haraguchi T, Hiraoka Y
  • Organizer: EMBO conference o Meiosis
  • Place of Presentation: Hotel Radisson Blu, Radeveul-Dresden, Germany
• [Presentation] Fission yeast nuclear membrane proteins that affect chromosome movements.
  • Author(s): Tange Y, Asakawa H, Yang HJ, Chikashige Y, Haraguchi T, Hiraoka Y
  • Organizer: The seventh international fission yeast meeting
  • Place of Presentation: Senate House, London, UK
  • Invited
• [Presentation] 染色体安定性に関わる分裂酵母核膜タンパク質
  • Author(s): 丹下喜恵、淺川東彦、原口徳子、平岡泰
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸市
  • Invited
• [Presentation] Meiotic nuclear movement in fission yeast is prolonged by stalled DNA replication through a Cds1-dependent checkpoint pathway.
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Haraguchi T, Masukata H, Hiraoka Y.
  • Organizer: The seventh international fission yeast meeting
  • Place of Presentation: Senate House, London, UK
• [Book] "Live CLEM imaging : an application for yeast cells". in : Current microscopy contributions to advances in science and technology, vol. 1 (2012)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Publisher: Formatex Research Center (Badajoz, Spain)
• [Book] Current microscopy contributions to advances in science and technology vol.1 (2012)
  • Author(s): Asakawa H
  • Publisher: Formatex Research Center
• [Remarks] 大阪大学大学院生命機能研究科 細胞核 ダイナミクス研究室
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/
• [Remarks] Hiraoka Laboratory
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html
• [Remarks] 大阪大学大学院生命機能研究科 細胞核ダイナミクス研究室
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/
• [Remarks] Hiraoka Laboratory
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html
• [Remarks] 大阪大学大学院生命機能研究科 細胞核ダイナミクス研究室
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/
• [Remarks] Hiraoka Laboratory
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html