Comparative phospho-motif analysis reveals the evolutionary expansion of phosphorylation signaling networks
| Project/Area Number |
24770190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | リン酸化 / シグナル伝達 / 比較進化解析 / モチーフ解析 / リン酸化シグナル / リン酸化モチーフ / 比較ゲノム解析 / 細胞内情報伝達 |
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| Outline of Final Research Achievements |
Protein phosphorylation is a post-translational modification that is essential for a wide range of eukaryotic physiological processes. However the physiological roles of most remain unknown. In this study, we developed a method to extract phosphosites with important roles in cellular functions.
We determined 178 phosphomotifs based on the analysis of 34,366 phosphosites. Comparative genomic analyses were performed using genomes from nine species from yeast to humans. Consequently, we identified 16 phosphomotifs, in which the level of conservation increased among species. The highly conserved phosphomotifs in humans and the worm were kinase regulatory sites. The motifs that appeared in the fly were novel phosphomotifs, including zinc finger motifs. The motifs that appeared in fish allowed the detection of the expansion of phosphorylation signaling related to alternative splicing.
Our method can be helpful for extracting novel phosphomotifs with physiological functions.
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Report
(4 results)
Research Products
(8 results)
